Dynamic Variations in Brain Glycogen are Involved in Modulating Isoflurane Anesthesia in Mice

作     者：Ze Fan Zhihao Zhang Shiyi Zhao Yuanyuan Zhu Dong Guo Bo Yang Lixia Zhuo Jiao Han Rui Wang Zongping Fang Hailong Dong Yan Li Lize Xiong Ze Fan;Zhihao Zhang;Shiyi Zhao;Yuanyuan Zhu;Dong Guo;Bo Yang;Lixia Zhuo;Jiao Han;Rui Wang;Zongping Fang;Hailong Dong;Yan Li;Lize Xiong

作者机构：Department of Anesthesiology and Perioperative MedicineXijing HospitalFourth Military Medical UniversityXi'an 710032ShaanxiChina College of Basic MedicineFourth Military Medical UniversityXi'an 710032ShaanxiChina Department of NeurobiologyFourth Military Medical UniversityXi'an 710032ShaanxiChina Center for Brain Science and Department of AnesthesiologyThe First Affiliated Hospital of Xi'an Jiaotong UniversityXi*an 710061ShaanxiChina Translational Research Institute of Brain and Brain-Like IntelligenceDepartment of Anesthesiology and Perioperative MedicineShanghai Fourth People's Hospital Affiliated to Tongji University School of MedicineShanghai 200081China 

出 版 物：《Neuroscience Bulletin》 (神经科学通报（英文版）)

年 卷 期：2020年第36卷第12期

页      面：1513-1523页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100217[医学-麻醉学] 10[医学] 

基　　金：the Major Program of the National Natural Science Foundation of China(81590954) the International Cooperation and Exchange of the National Natural Science Foundation of China(81420108013) the State Key Program of National Natural Science Foundation of China(81730032) 

主　　题：Anesthesia-arousal Brain glycogen General anesthesia Glycogen phosphorylase Glycogen synthetase Isoflurane 

摘      要：General anesthesia severely affects the metabolites in the ***,principally stored in astrocytes and providing the short-term delivery of substrates to neurons,has been implicated as an affected ***,whether glycogen plays a pivotal role in modulating anesthesia-arousal remains ***,we demonstrated that isoflurane-anesthetized mice exhibited dynamic changes in the glycogen levels in various brain *** synthase(GS)and glycogen phosphorylase(GP),key enzymes of glycogen metabolism,showed increased activity after isoflurane *** blocking glycogenolysis with 1,4-dideoxy-1,4-imino-Darabinitol(DAB),a GP antagonist,we found a prolonged time of emergence from anesthesia and an enhancedδfrequency in the EEG(electroencephalogram).In addition,augmented expression of glycogenolysis genes in glycogen phosphorylase,brain(Pygb)knock-in(PygbH11/H11)mice resulted in delayed induction of anesthesia,a shortened emergence time,and a lower ratio of EEG-δ.Our findings revealed a role of brain glycogen in regulating anesthesiaarousal,providing a potential target for modulating anesthesia.