Enhanced osteogenic differentiation of human periodontal ligament stem cells by suberoylanilide hydroxamic acid
作者机构：Department of StomatologyAffiliated Hospital of Yanbian UniversityYanji133000China Department of Immunology and Molecular MicrobiologySchool of Dentistry and Dental Research InstituteSeoul National UniversitySeoul08826South Korea Affiliated Hospital of Yanbian UniversityYanji133000China Department of Cell Biology and Medical GeneticsYanbian University Medical CollegeYanji133000China Department of Biosystems EngineeringKangwon National UniversityChuncheon24341South Korea
出 版 物：《BIOCELL》 (生物细胞（英文）)
年 卷 期：2020年第44卷第3期
页 面：389-400页
核心收录：
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：the National Research Foundation of Korea(NRF)and funded by the Ministry of Education(No.2018R1A6A1A03025582) the National Research Foundation of Korea(NRF-2019R1D1A3A03103828)
主 题：Suberoylanilide hydroxamic acid Histone deacetylase Osteoblast hPDLSCs Alkaline phosphatase Periodontal bone regeneration
摘 要：Periodontitis is a type of chronic inflammation in the gingival tissue caused by infectious bacteria colonizing the surface of the teeth,leading to the destruction of tooth-supporting tissues and loss of alveolar *** hydroxamic acid(SAHA),a class of histone deacetylase(HDAC)inhibitor,has the potential to stimulate osteoblast differentiation by acetylating histone proteins,and thus suppressing the expression of adipogenic and chondrogenic ***,the effect of SAHA on the differentiation of human periodontal ligament stem cells(hPDLSCs)is yet to be ***,we investigated the effects of SAHA on in vitro proliferation and differentiation of hPDLSCs by MTT assay,Alizarin Red-S,and alkaline phosphatase staining,and real-time ***,300 ng/mL SAHA treatment enhanced the proliferation and mineralization of hPDLSCs,indicating their osteogenic ***,a significant enhancement of osteogenesis gene markers and proteins was *** also demonstrated that ERK is a positive regulator of Runx2 transcription factors during osteoblast *** results indicate that SAHA may be a useful osteogenic induction agent for periodontal bone regeneration.
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