Transgene expression and differentiation of baculovirus-transduced adipose-derived stem cells from dystrophin-utrophin double knock-out mouse

作     者：Qiuling Li Qiongxiang Zhai Jia Geng Hui Zheng Fei Chen Jie Kong Cheng Zhang 

作者机构：Department of Neurology FirstAffiliated Hospital Sun Yat-sen University Guangzhou 510080 Guangdong Province China Department of Pediatrics Guangdong General Hospital Guangdong Neuroscience Institute Guangdong Academy of Medical Sciences Guangzhou 510080 Guangdong Province China Department of Neurology First Affiliated Hospital Kunming Medical College Kunming 650032 Yunnan Province China Department of Neurology Nanfang Hospital Southern Medical University Guangzhou 510515 Guangdong Province China Center for Stem Cell Biology and Tissue Engineering Sun Yat-sen University Guangzhou 510085 Guangdong Province China 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2012年第7卷第22期

页      面：1695-1702页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 0711[理学-系统科学] 090403[农学-农药学(可授农学、理学学位）] 07[理学] 08[工学] 09[农学] 0904[农学-植物保护] 

基　　金：supported by the National Natural Science Foundation of China,No.30370510,30170337,30400322,30870851 CMB Fund,No.4209347 Key Project of the State Ministry of Public Health,No.2001321 Fok Ying Tung Education Foundation,No.91029 Key Projects in the National Science and Technology Pillar Program During the Eleventh Five-Year Plan Period,No.2006BAI05A07 

主　　题：baculovirus adipose-derived stem cells Duchenne muscular dystrophy microdystrophin β-catenin myogenesis gene therapy neural regeneration 

摘      要：In this study, recombinant baculovirus carrying the microdystrophin and β-catenin genes was used to infect adipose-derived stem cells from a dystrophin-utrophin double knock-out mouse. Results showed that, after baculovirus transgene infection, microdystrophin and β-catenin genes were effectively expressed in adipose-derived stem cells from the dystrophin-utrophin double knock-out mouse. Furthermore, this transgenic expression promoted adipose-derived stem cell differentiation into muscle cells, but inhibited adipogenic differentiation. In addition, protein expression related to the microdystrophin and Wnt/β-catenin signaling pathway was upregulated. Our experimental findings indicate that baculovirus can successfully deliver the microdystrophin and β-catenin genes into adipose-derived stem cells, and the microdystrophin and Wnt/β-catenin signaling pathway plays an important role in myogenesis of adipose-derived stem cells in the dystrophin-utrophin double knock-out mouse.