Phytohormone dynamics in developing endosperm influence rice grain shape and quality

作     者：Xiao-Fan Zhang Jian-Hua Tong Ai-Ning Bai Chun-Ming Liu Lang-Tao Xiao Hong-Wei Xue Xiao-Fan Zhang;Jian-Hua Tong;Ai-Ning Bai;Chun-Ming Liu;Lang-Tao Xiao;Hong-Wei Xue

作者机构：School of Life Science and TechnologyShanghai Tech UniversityShanghai 201210China Hunan Provincial Key Laboratory of Phytohormones and Growth DevelopmentHunan Agricultural UniversityChangsha 410128China Key Laboratory of Plant Molecular PhysiologyInstitute of Botanythe Chinese Academy of SciencesBeijing 100093China Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China Joint Center for Single Cell Biology/School of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China National Key Laboratory of Plant Molecular GeneticsCAS Center for Excellence in Molecular Plant SciencesShanghai Institute of Plant Physiology and Ecologythe Chinese Academy of SciencesShanghai 200032China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2020年第62卷第10期

页      面：1625-1637页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 

基　　金：Funding for this research is gratefully acknowledged fromthe National Key Research and Development Programof China(2016YFD0100501,2016YFD0100902) the National Transformation Science and Technology Pro-gram(2016ZX08001006-009). 

主　　题：fir grain shape 

摘      要：Hormones are important signaling molecules regulating developmental processes and responses to environmental stimuli in higher plants.Rice endosperm,the portion of the seed surrounding the embryo,is the main determinant of rice grain shape and yield;how-ever,the dynamics and exact functions of phyto-hormones in developing endosperm remain elusive.Through a systemic study including transcriptome analysis,hormone measurement,and transgene-based endosperm-specific expression of phytohormone bio-synthetic enzymes,we demonstrated that dynamic phytohormone levels play crucial roles in the developing rice endosperm,particularly in regard to grain shape and quality.We detected diverse,differential,and dra-matically changing expression patterns of genes related to hormone biosynthesis and signaling during endosperm development,especially at early devel-opmental stages.Liquid chromatography measure-ments confirmed the dynamic accumulation of hor-mones in developing endosperm.Further transgenic analysis performed on plants expressing hormone bio-synthesis genes driven by an endosperm-specific pro-moter revealed differential effects of the hormones,especially auxin and brassinosteroids,in regulating grain shape and quality.Our studies help elucidate the dis-tinct roles of hormones in developing endosperm and provide novel and useful tools for influencing crop seed shape and yield.