Extension and application of a database for the rapid identification of Vibrio using MALDI-TOF MS

作     者：Jing Wu Yuexia Zhou Xiaofei Liu Yuan Cao Chengjin Hu Yingjian Chen Jing Wu;Yuexia Zhou;Xiaofei Liu;Yuan Cao;Chengjin Hu;Yingjian Chen

作者机构：Departments of Laboratory MedicineThe 960th Hospital of the PLA Joint Logistics Support ForceJinan 250031China Teaching and Research Office of Medical Laboratory ScienceHeze Medical CollegeHeze 274000China 

出 版 物：《Acta Oceanologica Sinica》 (海洋学报（英文版）)

年 卷 期：2020年第39卷第10期

页      面：140-146页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1001[医学-基础医学(可授医学、理学学位)] 0906[农学-兽医学] 09[农学] 100103[医学-病原生物学] 10[医学] 

基　　金：Foundation item:The Major PLA Research Project of“the 12th Five-year Plan”for Medical Science Develpment under contract No.BWS12J014 

主　　题：database Vibrio MALDI-TOF MS rpoB dendrograms 

摘      要：Rapid and accurate identification of Vibrio species has been problematic because phenotypic characteristics are variable within species and biochemical identification requires two or more days to ***-assisted laser desorption ionization–time of flight mass spectrometry(MALDI-TOF MS)has become a powerful tool for rapidly distinguishing between related bacterial ***,its accuracy depends on the number of strains in a *** the current study,we extend and apply the Vibrio database based on MALDI-TOF MS.A total of 74 strains of Vibrio representing 28 species were identified and included in new database.A phylogenetic tree based on rpoB sequence and dendrograms were *** analyzed 30 clinical Vibrio of three species to evaluate the new database and carried out PCA dendrogram analyses for differences of *** created a new database that offered fast and accurate Vibrio *** and PCA dendrogram analyses provided technical support to track sources and incidences of Vibrio *** addition,the discovery of characteristic and differential peaks is useful for the future identification of *** represents a powerful tool for the rapid and accurate classification and identification of Vibrio and closely related species.