Genetic variation and phylogenetic relationship of Hypoderaeum conoideum(Bloch, 1782) Dietz, 1909(Trematoda: Echinostomatidae) inferred from nuclear and mitochondrial DNA sequences

作     者：Chairat Tantrawatpan Weerachai Saijuntha Chairat Tantrawatpan;Weerachai Saijuntha

作者机构：Division of Cell BiologyDepartment of Preclinical SciencesFaculty of MedicineThammasat UniversityRangsit CampusPathumthani 12120Thailand Walai Rukhavej Botanical Research InstituteMahasarakham UniversityMaha Sarakham 44150Thailand 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2020年第13卷第11期

页      面：515-520页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 07[理学] 0905[农学-畜牧学] 09[农学] 071007[理学-遗传学] 090501[农学-动物遗传育种与繁殖] 

基　　金：supported by Faculty of Medicine Thammasat University Thailand to CT grant number 2-18/2562 

主　　题：Echinostomes Genetic diversity Genetic differentiation Nuclear ribosomal DNA Cytochrome c oxidase subunit 1 NADH dehydrogenase subunit 1 

摘      要：Objective:To explore genetic variations of Hypoderaeum conoideum collected from domestic ducks from 12 different localities in Thailand and Lao PDR,as well as their phylogenetic relationship with American and European ***:The nucleotide sequences of their nuclear ribosomal DNA(ITS),mitochondrial cytochrome c oxidase subunit 1(CO1),and NADH dehydrogenase subunit 1(ND1)were used to analyze genetic diversity ***:We found relatively high levels of nucleotide polymorphism in ND1(4.02%),whereas moderate and low levels were observed in CO1(2.11%)and ITS(0.96%),*** on these polymorphisms,the 20 ND1,12 CO1,and 18 ITS haplotypes were classified,and several common haplotypes were observed in all *** least three major lineages,namely American,European and Asian lineages,have been classified by phylogenetic analyses based on ND1 ***:Our report demonstrates that the ND1 gene is the most suitable genetic marker to explore genetic variation and phylogenetic relationship of Hypoderaeum ***,a combination of all loci for ND1,CO1 and ITS would be of great value toward further genetic investigation of this endemic worldwide ***,comprehensive molecular genetic analyses of Hypoderaeum conoideum from its worldwide distribution is needed to further understanding of the evolutionary and systematic relationships of this parasite.