Targeted Gene Knockouts Reveal Overlapping Functions of the Five Physcomitrella patens FtsZ Isoforms in Chloroplast Division, Chloroplast Shaping, Cell Patterning, Plant Development, and Gravity Sensing
Targeted Gene Knockouts Reveal Overlapping Functions of the Five Physcomitrella patens FtsZ Isoforms in Chloroplast Division, Chloroplast Shaping, Cell Patterning, Plant Development, and Gravity Sensing作者机构：Plant Biotechnology Faculty of Biology University of Freiburg Schaenzlestr. 1 79104 Freiburg Germany Centre for Biological Signalling Studies (bioss) University of Freiburg Alberststr. 19 79104 Freiburg Germany Spemann Graduate School of Biology and Medicine (SGBM) University of Freiburg Alberststr. 19A 79104 Freiburg Germany
出 版 物：《Molecular Plant》 (分子植物（英文版）)
年 卷 期：2009年第2卷第6期
页 面：1359-1372页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 090102[农学-作物遗传育种]
基 金：德国德意志研究联合会(DFG)资助 Excellence Initiative of the German Federal and State Governments
主 题：Bryophyte cell wall gravitropism GTPase chloroplast plastoskeleton P patens moss.
摘 要：Chloroplasts and bacterial cells divide by binary fission. The key protein in this constriction division is FtsZ, a self-assembling GTPase similar to eukaryotic tubulin. In prokaryotes, FtsZ is almost always encoded by a single gene, whereas plants harbor several nuclear-encoded FtsZ homologs. In seed plants, these proteins group in two families and all are exclusively imported into plastids. In contrast, the basal land plant Physcomitrella patens, a moss, encodes a third FtsZ family with one member. This protein is dually targeted to the plastids and to the cytosol. Here, we report on the targeted gene disruption of all ftsZ genes in R patens. Subsequent analysis of single and double knockout mutants revealed a complex interaction of the different FtsZ isoforms not only in plastid division, but also in chloroplast shaping, cell patterning, plant development, and gravity sensing. These results support the concept of a plastoskeleton and its functional integration into the cytoskeleton, at least in the moss R patens.
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