SALT-RESPONSIVE ERF1 Is a Negative Regulatorof Grain Filling and Gibberellin-MediatedSeedling Establishment in Rice

作     者：Romy Schmidt Jos H. M. Schippers Delphine Mieulet 

作者机构：Institute of Biochemistry and Biology University of Potsdam Max Planck Institute of Molecular Plant Physiology 

出 版 物：《Molecular Plant》 (分子植物（英文版）)

年 卷 期：2014年第7卷第2期

页      面：404-421页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 0901[农学-作物学] 0902[农学-园艺学] 

基　　金：supported by grants from the Federal Ministry of Education and Research (BMBF) in Germany the Agence Nationale de la Recherche (ANR) in France the RicE Functional Genomics (REFUGE) platform (Montpellier France) funded by Agropolis Fondation funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript 

主　　题：RPBF rice grain filling germination SERF1 gibberellic acid 

摘      要：Grain quality is an important agricultural trait that is mainly determined by grain size and ***, we characterize the role of the rice transcription factor （TF） SALT-RESPONSIVE ERF1 （SERF1） during grain develop-ment. Through genome-wide expression profiling and chromatin immunoprecipitation, we found that SERF1 directlyregulates RICE PROLAMIN-BOX BINDING FACTOR （RPBF）, a TF that functions as a positive regulator of grain filling. Lossof SERF1 enhances RPBF expression resulting in larger grains with increased starch content, while SERF1 overexpressionrepresses RPBF resulting in smaller grains. Consistently, during grain filling, starch biosynthesis genes such as GRANULE-BOUND STARCH SYNTHASEI （GBSSI）, STARCH SYNTHASEI （SSI）, SSIIla, and ADP-GLUCOSE PYROPHOSPHORYLASELARGE SUBUNIT2 （AGPL2） are up-regulated in SERF1 knockout grains. Moreover, SERF1 is a direct upstream regulator ofGBSSI. In addition, SERF1 negatively regulates germination by controlling RPBF expression, which mediates the gibberel-lic acid （GA）-induced expression of RICE AMYLASEIA （RAmylA）. Loss of SERF1 results in more rapid seedling establish-ment, while SERF1 overexpression has the opposite effect. Our study reveals that SERF1 represents a negative regulatorof grain filling and seedling establishment by timing the expression of RPBF.