Loss of scleraxis leads to distinct reduction of mineralized intermuscular bone in zebrafish

作     者：Chunhong Nie Shiming Wan Yulong Chen Dejie Zhu Xudong Wang Xiaoru Dong Ze-Xia Gao 

作者机构：College of FisheriesKey Laboratory of Freshwater Animal BreedingMinistry of Agriculture and Rural Affair/Key Laboratory of Agricultural Animal GeneticsBreeding and ReproductionMinistry of EducationHuazhong Agricultural UniversityWuhanHubei430070China Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic BeltMinistry of EducationWuhan430070China Hubei Engineering Technology Research Center for Fish Breeding and Healthy AquacultureWuhan430070China 

出 版 物：《Aquaculture and Fisheries》 (渔业学报（英文）)

年 卷 期：2021年第6卷第2期

页      面：169-177页

学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 0908[农学-水产] 0707[理学-海洋科学] 0906[农学-兽医学] 09[农学] 0713[理学-生态学] 

基　　金：This work was financially supported by the National Natural Science Foundation of China(Grant No.31872559) National Key Research and Development Program(Grant No.2018YFD0900102) Modern Agriculture Industry Technology System Construction Projects of China titled as-Staple Freshwater Fishes Industry Technology System(Grant No.CARS-46-08) Fundamental Research Funds for the Central Universities(Grant No.2662018PY035) State Key Laboratory of Developmental Biology of Freshwater Fish(2018KF003) 

主　　题：Intermuscular bone Scleraxis CRISPR-Cas9 Phenotype Gene expression Gene function 

摘      要：Intermuscular bones(IBs)are ossified from tendons and only occur in lower *** association between the regulation of scleraxis gene(scx)and tendon development gave us reasons to speculate that the scx gene may play a potential role in regulating the development of IBs.A phylogenetic analysis conducted for this study revealed potential functional differentiation between two scx orthologues,scxa and *** scxa^(-/-) and scxb^(-/-) zebrafish were generated through CRISPR-Cas9 technology to study the role of scx in the IB and rib *** results showed a significant reduction of the number of IBs in adult scxa-1^(-/-) zebrafish,with almost 70%reduction(15-25 IBs)compared to the wild type scxa-1^(+/+)zebrafish(76-80 IBs).In the scxa-1^(+/+)adults,IBs were observed in both dorsal and tail segments;however,in scxa-1^(-/-)fish IBs were observed only in the tail segment(none in the dorsal segment).Although scxa-1^(-/-) zebrafish had rib defects,the mutants were viable and fertile as adult *** scxb^(-/-) zebrafish had the same number of IBs and same skeletal phenotype as the wild-type *** suggests that only scxa has a crucial role in the IB development,and confirms functional differentiation of scx *** further clarify the molecular mechanism by which scxa affects the IB development,we conducted comparative transcriptome analysis of dorsal tissue samples of scxa-1^(+/+)(with IBs)and scxa-1^(-/-) (without IBs),and further verified the expression of key genes via *** is the first study to identify a gene that controls the amount of IBs in fish,and it provides a new sight into the effects of scxa on the molecular mechanism of IB development in fish.