Elevation of gene expression of calcineurin,calmodulin and calsyntenin in oxidative stress induced PC12 cells

作     者：P.Aravind Sarojini R.Bulbule N.Hemalatha R.L.Babu K.S.Devaraju 

作者机构：Department of BiochemistryKarnatak UniversityPavate NagarDharwad580003India Department of Biochemistry and NutritionCFTRIV V MohollaMysore570020India Department of Bioinformatics and BiotechnologyAkkamahadevi Women’s UniversityVijayapuraKarnataka586108India 

出 版 物：《Genes & Diseases》 (基因与疾病（英文）)

年 卷 期：2021年第8卷第1期

页      面：87-93页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 071010[理学-生物化学与分子生物学] 1002[医学-临床医学] 081704[工学-应用化学] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 08[工学] 0817[工学-化学工程与技术] 0703[理学-化学] 

基　　金：DST-SERB New Delhi for providing financial Grant(SB/EMEQ-238/2013)to Dr.K.S.Devaraju.Aravind.P greatly acknowledges the University Research fellowship sanctioned by Karnatak University Dharwad 

主　　题：Akt Calcineurin Calmodulin Oxidative stress Peroxynitrite 

摘      要：In normal physiological conditions,reactive oxygen and nitrogen species are used as important signaling molecules in the ***,in excess it causes the disruption of cell resulting in their *** stress causes influx in intracellular calcium levels leading to higher concentrations of calcium in the *** accelerated calcium affects both the mitochondria and nuclei leading to excitotoxicity in *** calcium levels are controlled by voltage dependent calcium channels located in the plasma membrane,calcium stores like endoplasmic/sarcoplasmic reticulum and majorly by calcium binding *** study was aimed at analyzing the gene expression of major calcium binding proteins namely calcineurin,calmodulin,calreticulin,synaptotagamin and calsyntenin in stress induced PC 12 ***(1 mM),Peroxynitrite(10 mM),H_(2)O_(2)(100 mM)and High glucose(33 mM)were used to induce oxidative stress in PC12 *** obtained from the study suggest that calcineurin,calmodulin and calsyntenin gene expression were enhanced compared to the control due to oxidative ***,synaptotagmin and calreticulin gene expression were down ***,Akt protein expression(stress marker)was enhanced in PC12 cells with all other stress inducers except in hyperglycemic condition.