Oil-mediated high-throughput generation and sorting of water-in-water droplets
作者机构：Department of Mechanical EngineeringThe University of Hong KongPokfulam RoadHong KongChina Department of Electrical and Electronic EngineeringThe University of Hong KongPokfulam RoadHong KongChina
出 版 物：《Microsystems & Nanoengineering》 (微系统与纳米工程（英文）)
年 卷 期：2020年第6卷第1期
页 面：484-493页
核心收录：
学科分类：0711[理学-系统科学] 07[理学] 081104[工学-模式识别与智能系统] 08[工学] 0811[工学-控制科学与工程] 071102[理学-系统分析与集成] 081103[工学-系统工程]
基 金：This work was supported by the General Research Fund(Nos.17307919,17329516,17304017,17305518,and 17306315) the Research Impact Fund(No.R7072-18)from the Research Grants Council of Hong Kong,Excellent Young Scientists Fund(Hong Kong and Macao)(21922816) the National Natural Science Foundation of China(NSFC)as well as the Seed Fund for Basic Research(Nos.201811149241,201711159249,and 201611159205) the Seed Fund for Translational and Applied Research(No.201711160016) Platform Technology Funding from the University of Hong Kong
主 题：limiting sized compatibility
摘 要：Aqueous two-phase system(ATPS)droplets have demonstrated superior compatibility over conventional water-in-oil droplets for various biological ***,the ultralow interfacial tension hampers efficient and stable droplet generation,limiting further development and more extensive use of such ***,we present a simple strategy to employ oil as a transient medium for ATPS droplet *** methods based on passive flow focusing and active pico-injection are demonstrated to generate water-water-oil double emulsions,achieving a high generation frequency of~2.4 *** evaporation of the oil to break the double emulsions,the aqueous core can be released to form uniform-sized water-in-water ***,this technique can be used to fabricate aqueous microgels,and the introduction of the oil medium enables integration of droplet sorting to produce single-cell-laden hydrogels with a harvest rate of over 90%.We believe that the demonstrated high-throughput generation and sorting of ATPS droplets represent an important tool to advance droplet-based tissue engineering and single-cell analyses.
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