A comparison between chemical and gas hypoxia as models of global ischemia in zebrafish (Danio rerio)

作     者：Kaitlyn M.Marino Emani R.Silva James A.Windelborn Kaitlyn M.Marino;Emani R.Silva;James A.Windelborn

作者机构：Department of BiologyWashington CollegeChestertownMDUSA Department of PsychologyWashington CollegeChestertownMDUSA 

出 版 物：《Animal Models and Experimental Medicine》 (动物模型与实验医学（英文）)

年 卷 期：2020年第3卷第3期

页      面：256-263页

核心收录：

学科分类：1002[医学-临床医学] 100204[医学-神经病学] 10[医学] 

主　　题：hypoxia hypoxic-ischemia ischemia 

摘      要：Background:Zebrafish models for neurovascular diseases offer new methods for elucidation of molecular pathways to tissue *** fertilization and high fecundity provide opportunities for transgenics and other forms of genetic manipulation that are more accessible than offered by mammalian models of ***,behavioral analyses of zebrafish allow for connection of molecular pathways to organismal outputs such as locomotion,learning,and ***,a zebrafish model of hypoxia-ischemia has been slow to catch on,possibly due to hypoxia exposure protocols that are challenging to reproduce and result in high ***:In this study,we have introduced a predictable and simple method of hypoxia induction,the addition of sodium sulfite to aquarium *** effects of this treatment on zebrafish locomotion were compared to those of zebrafish exposed to hypoxia induced by nitrogen gas bubbling,a method used in previous ***:We found that hypoxia induced by sodium sulfite significantly impaired locomotion in the hours following treatment,and its effects did not differ from those caused by nitrogen gas ***:These results indicate that hypoxia by sodium sulfite represents an effective and easily reproducible method for the study of hypoxia-ischemia in zebrafish.