Multienzyme co-immobilization-based bioelectrode:Design of principles and bioelectrochemical applications

作     者：Ranran Wu Haiyan Song Yuanming Wang LeiWang Zhiguang Zhu Ranran Wu;Haiyan Song;Yuanming Wang;Lei Wang;Zhiguang Zhu

作者机构：Tianjin Institute of Industrial BiotechnologyChinese Academy of Sciences32West Seventh AvenueTianjin Airport Economic AreaTianjin 300308China University of Chinese Academy of Sciences19A Yuquan RoadShijingshan DistrictBeijing 100049China National Human Genetic Resource Center12 Dahuisi RoadHaidian DistrictBeijing 100081China 

出 版 物：《Chinese Journal of Chemical Engineering》 (中国化学工程学报（英文版）)

年 卷 期：2020年第28卷第8期

页      面：2037-2050页

核心收录：

学科分类：081704[工学-应用化学] 081705[工学-工业催化] 08[工学] 0817[工学-化学工程与技术] 080502[工学-材料学] 0805[工学-材料科学与工程（可授工学、理学学位）] 

基　　金：supported by the National Natural Science Foundation of China(21878324,21706273) the CAS Pioneer Hundred Talent Program(Type C,reference#2016-081) 

主　　题：Multienzyme co-immobilization Bioelectrode Bioelectrochemical system Cascade reaction 

摘      要：Enzyme cascade reactions play significant roles in bioelectrochemical processes because they permit more complex reactions. Co-immobilization of multienzyme on the electrode could help to facilitate substrate/intermediate transfer among different enzymes and electron transfer from enzyme active sites to the electrode with high stability and retrievability. Different co-immobilization strategies to construct multienzyme bioelectrodes have been widely reported, however, up to now, they have barely been reviewed. In this review, we focus on recent state-of-the-art techniques for constructing co-immobilized multienzyme electrodes including random and positional co-immobilization. Particular attention is given to strategies such as multienzyme complex and surface display. Cofactor co-immobilization on the electrode is also crucial for the enhancement of catalytic reaction and electron transfer, yet, few studies have been reported. The up-to-date advances in bioelectrochemical applications of multienzyme bioelectrodes are also presented. Finally, key challenges and future perspectives are discussed.