Cryo-copolymerization preparation of dextran-hyaluronate based supermacroporous cryogel scaffolds for tissue engineering applications

作     者：Dongjiao ZHOU Shaochuan SHEN Junxian YUN Kejian YAO Dong-Qiang LIN Dongjiao ZHOU;Shaochuan SHEN;Junxian YUN;Kejian YAO;Dong-Qiang LIN

作者机构：State Key Laboratory Breeding Base of Green Chemistry Synthesis TechnologyCollege of Chemical Engineering and Materials ScienceZhejiang University of TechnologyHangzhou 310032China Department of Chemical and Biological EngineenngZhejiang UniversityHangzhou 310027China 

出 版 物：《Frontiers of Chemical Science and Engineering》 (化学科学与工程前沿（英文版）)

年 卷 期：2012年第6卷第3期

页      面：339-347页

核心收录：

学科分类：081702[工学-化学工艺] 08[工学] 0817[工学-化学工程与技术] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 

基　　金：Acknowledgements This work was supported by the National Natural Science Foundation of China (Grant Nos. 20876145  21036005)  the Science and Technology Cooperation Project between China-Europe Country's Governments from the Ministry of Science and Technology of China (No. 1017) and the Zhejiang Provincial Natural Science Foundation of China (No. Y4080326) 

主　　题：cryogel scaffold tissue engineering dextran,hyaluronate 3T3-L1 preadipocyte 

摘      要：Dextran-hyaluronate （Dex-HA） based supermacroporous cryogel scaffolds for soft tissue engineering were prepared by free radical cryo-copolymerization of aqueous solutions containing the dextran methacrylate （Dex-MA） and hyaluronate methacrylate （HA-MA） at various macromonomer concentrations under the freezing condition. It was observed that the suitable total concen- tration of macromonomers for the preparation of Dex-HA cryogel scaffold with satisfied properties was 5% （w/w） at the HA-MA concentration of 1% （w/v）, which was then used to produce the test scaffold. The obtained cryogel scaffold with 5% （w/w） macromonomer solution had high water permeability （5.1 × 10 ^-2m2） and high porosity （92.4%）. The pore diameter examined by scanning electron microscopy （SEM） was in a broad range of 50-135 um with the mean pore diameter of 91 um. Furthermore, the cryogel scaffold also had good elastic nature with the elastic modulus of 17.47±1.44 kPa. The culture of 3T3-L1 preadipocyte within the scaffold was investigated and observed by SEM. Cells clustered on the pore walls and grew inside the scaffold indicating the Dex-HA cryogel scaffold could be a promising porous biomaterial for applications in tissue engineering.