Photosensitive Azopolymer Brushes via Atom Transfer Radical Polymerization for Protein Sensing

作     者：Zhang, Zhihong Wang, Yaoli Yan, Fufeng Peng, Donglai Ma, Zhi 

作者机构：Henan Provincial Key Laboratory of Surface & Interface Science Zhengzhou University of Light Industry Zhengzhou Henan 450002 China Shanghai Institute of Organic Chemistry Chinese Academy of Sciences Shanghai 200032 China 

出 版 物：《Chinese Journal of Chemistry》 (中国化学（英文版）)

年 卷 期：2011年第29卷第1期

页      面：153-158页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070305[理学-高分子化学与物理] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 

基　　金：河南省高校科技创新人才支持计划项目 International Cooperation Project of Henan Province 

主　　题：azopolymer photoisomerization atom transfer radical polymerization (ATRP) adsorption biosensor 

摘      要：Novel photosensitive azopolymer brushes were synthesized via surface initiated atom transfer radical polymerization using initiator self-assembled on Au surface. The chemical structures of azobenzene derivatives were confirmed by Fourier transform infrared spectroscopy （FTIR） and nuclear magnetic resonance spectroscopy （NMR）. The surface morphology of azopolymers via atom transfer radical polymerization （ATRP） for different time was investigated by atomic force microscopy （AFM）. Additionally, the photoisomerization of azopolymer was measured by ultraviolet-visible spectroscopy （UV-Vis）, The results indicate that such azopolymers can undergo trans-cis-trans photoisomerization efficiently by photo-irradiation with UV light. Furthermore, this photoisomerization property could also induce the reversible adsorption of bovine serum albumin （BSA） adsorption on azopolymer brush surfaces. This adsorption kinetics of the reversible process can be measured by surface plasmon resonance （SPR） spectroscopy in situ. It suggests that the protein biochips could be regenerated safely by UV irradiation rather than by being rinsed with chemical reagents.