Inhibition of GABAA-ρreceptors induces retina regeneration in zebrafish

作     者：Matthew R.Kent Nergis Kara James G.Patton Matthew R. Kent;Nergis Kara;James G. Patton

作者机构：Department of Biological SciencesVanderbilt UniversityNashvilleTNUSA 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2021年第16卷第2期

页      面：367-374页

核心收录：

学科分类：1002[医学-临床医学] 100212[医学-眼科学] 10[医学] 

基　　金：grants from the NIH R01EY024354-S1 and UO1 EY027265 to JGP and T32 EY021453 additional support from the Stevenson family and Gisela Mosig endowments to Vanderbilt University 

主　　题：gamma aminobutyric acid morpholino Müller glia neurotransmitter regeneration retina stem cells zebrafish 

摘      要：A potential treatment for retinal diseases is to induce an endogenous Müller glia(MG)-derived regenerative response to replace damaged *** contrast to mammalian MG,zebrafish MG are capable of mediating spontaneous *** seek to define the mechanisms that enable retina regeneration in zebrafish in order to identify therapeutic targets to induce mammalian retina *** previously used pharmacological and genetic methods to inhibit gamma aminobutyric acid A(GABAA)receptors in undamaged zebrafish retinas and showed that such inhibition could induce initiation of retina regeneration,as measured by the dedifferentiation of MG and the appearance of MG-derived proliferating progenitor ***,we show that inhibition of a pharmacologically distinct subset of GABAA receptors(GABAA-ρ)can also induce retina *** inhibition of both GABA receptor subtypes led to enhanced retina *** expression analyses indicate that inhibition of GABAA-ρreceptors induces a canonical retinal regenerative *** results support a model in which decreased levels of GABA,such as would occur after retinal cell death or damage,induce dedifferentiation of MG and the generation of proliferating progenitor cells during zebrafish retina *** experiments were approved by the Vanderbilt s Institutional Animal Care and Use Committee(Protocol M1800200)on January 29,2019.