Neuroprotection by cattle encephalon glycoside and ignotin beyond the time window of thrombolysis in ischemic stroke

作     者：Jun Zhong Rong-Wei Li Ju Wang Ying Wang Hong-Fei Ge Ji-Shu Xian Hua Feng Liang Tan Jun Zhong;Rong-Wei Li;Ju Wang;Ying Wang;Hong-Fei Ge;Ji-Shu Xian;Hua Feng;Liang Tan

作者机构：Department of NeurosurgerySouthwest HospitalThe Third Military Medical University(Army Medical University)ChongqingChina Department of NeurosurgeryHanzhong Central HospitalHanzhongShaanxi ProvinceChina Department of OncologyHanzhong Central HospitalHanzhongShaanxi ProvinceChina State Key Laboratory of Power Transmission Equipment and System Security and New TechnologySchool of Electrical EngineeringChongqing UniversityChongqingChina 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2021年第16卷第2期

页      面：312-318页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100204[医学-神经病学] 10[医学] 

基　　金：the 63rd Batch of First-Class Financing for Chinese Postdoctoral Science Foundation,No.2018M631061(to LT) Macao Youth Scholars Program,No.AM201918(to LT) 

主　　题：apoptosis brain cattle encephalon glycoside and ignotin central nervous system neurological function plasticity rat regeneration stroke 

摘      要：Cattle encephalon glycoside and ignotin(CEGI)injection is known as a multi-target neuroprotective drug that contains numerous liposoluble molecules,such as polypeptides,monosialotetrahexosyl ganglioside(GM-1),free amino acids,hypoxanthine and *** has been approved by the Chinese State Food and Drug Administration and widely used in the treatments of various diseases,such as stroke and Alzheimer s ***,the neuroprotective effects of CEGI beyond the time window of thrombolysis(within 4.5 hours)on acute ischemic stroke remain *** study constructed a rat middle cerebral artery occlusion model by suture-occluded method to simulate ischemic *** first daily dose was intraperitoneally injected at 8 hours post-surgery and the CEGI treatments continued for 14 *** of the modified five-point Bederson scale,beam balance test and rotameric test showed the neurological function of ischemic stroke rats treated with 4 m L/kg/d CEGI improved significantly,but the mortality within 14 days did not change *** MRI and 2,3,5-triphenyltetrazolium chloride staining confirmed that the infarct size in the 4 m L/kg/d CEGI-treated rats was significantly reduced compared with ischemic insult *** results of transmission electron microscopy and double immunofluorescence staining showed that the hippocampal neuronal necrosis in the ischemic penumbra decreased whereas the immunopositivity of new neuronal-specific protein doublecortin and the percentage of Ki67/doublecortin positive cells increased in CEGI-treated rats compared with untreated *** results suggest that CEGI has an effective neuroprotective effect on ischemic stroke when administered after the time window of *** study was approved by the Animal Ethics Committee of The Third Military Medical University,China.