Progressive changes of orexin system in a rat model of 6-hydroxydopamine-induced Parkinson’s disease

作     者：崔龙彪 李博为 金晓航 赵琳 史娟 

作者机构：第四军医大学人体解剖与组织胚胎学教研室西安710032 第四军医大学航空航天医学系2006级本科西安710032 延安大学医学院人体解剖学教研室延安716000 

出 版 物：《Neuroscience Bulletin》 (神经科学通报（英文版）)

年 卷 期：2010年第26卷第5期

页      面：381-387页

核心收录：

学科分类：1002[医学-临床医学] 100204[医学-神经病学] 10[医学] 

基　　金：supported by the grant from National Natural Science Foundation of China(No.30871004 31071012) 

主　　题：orexin Parkinson’s disease sleep disturbance degeneration 

摘      要：Objective Sleep disturbance,which is characterized by excessive daytime sleepiness and sleep attacks,is frequently observed in patients with Parkinson’s disease（PD）.Loss of orexin neurons in hypothalamus and the resultant decreased level of orexin in cerebrospinal fluid（CSF）found in narcolepsy patients may also play an essential role in the pathogenesis of sleep *** present study aimed to investigate the possible changes in the orexin system during PD *** After the establishment of a rat PD model by injecting 6-hydroxydopamine（6-OHDA）into the medial forebrain bundle,the numbers of orexin-A-and tyrosine hydroxylase（TH）-positive neurons,and the levels of orexin-A fibers and orexin-A in CSF were examined by immunohistochemistry and ELISA assay,*** Compared to the TH-containing neurons that exhibited fast degeneration in response to 6-OHDA,orexin-A-containing neurons were less sensitive to *** number of orexin-A-positive neurons began to decrease at day 21 after operation,and at day 49,it decreased by 30%of the initial *** orexin-A level in CSF of PD rats did not show any obvious fluctuations compared to the control,and there was no obvious reduction in the density of orexin-A-positive fibers in brain areas such as tuberomammillary *** These results reveal for the first time the dynamic changes of orexin system during the progression of *** may provide valuable information for drug development to reverse the loss of orexin neurons and sleep disturbance in PD patients.