Immune Responses to Varicella-Zoster Virus Glycoprotein E Formulated with Poly(Lactic-co-Glycolic Acid) Nanoparticles and Nucleic Acid Adjuvants in Mice

作     者：Yunfei Wang Jialong Qi Han Cao Cunbao Liu Yunfei Wang;Jialong Qi;Han Cao;Cunbao Liu

作者机构：Institute of Medical BiologyChinese Academy of Medical Sciences and Peking Union Medical CollegeKunming 650118China 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2021年第36卷第1期

页      面：122-132页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100102[医学-免疫学] 10[医学] 

基　　金：financially supported by the CAMS Initiative for Innovative Medicine(Grant Number 2017-I2M3-022) Central basic scientific research in colleges and universities(Grant Number 3332019162) the National Natural Science Foundation of China(Grant Number 81503117) the Foundation for Studying Abroad from the China Scholarship Council(Grant Number 201808110121) 

主　　题：Varicella-zoster virus subunit vaccine Cell-mediated immunity Nucleic acid immune stimulator Poly I:C CpG ODN PLGA Nanoparticle 

摘      要：The subunit herpes zoster vaccine Shingrix is superior to attenuated vaccine Zostavax in both safety and efficacy,yet its unlyophilizable liposome delivery system and the limited supply of naturally sourced immunological adjuvant QS-21 still need to be *** on poly(lactic-co-glycolic acid)(PLGA)delivery systems that are stable during the lyophilization and rehydration process and using a double-emulsion(w/o/w)solvent evaporation method,we designed a series of nanoparticles with varicella-zoster virus antigen glycoprotein E(VZV-g E)as an antigen and nucleic acids including polyinosinic-polycytidylic acid(Poly I:C)and phosphodiester Cp G oligodeoxynucleotide(Cp G ODN),encapsulated as immune *** cationic lipids(DOTAP)have more potential than neutral lipids(DOPC)for activating g E-specific cell-mediated immunity(CMI)in immunized mice,especially when g E is encapsulated in and presented on the surface of nanoparticles,PLGA particles without lipids have the greatest potential to induce not only the highest g Especific Ig G titers but also the strongest g E-specific CMI responses,including the highest proportions of interferon-c(IFNc)-and interleukin-2(IL-2)-producing CD4?/CD8?T cells according to a flow cytometry assay and the greatest numbers of IFN-c-and IL-2-producing splenocytes according to an enzyme-linked immunospot(ELISPOT)*** results showed that immune-stimulating nucleic acids together with the PLGA delivery system showed promise as a safe and economical varicella and zoster vaccine candidate.