MicroRNA-184 negatively regulates corneal epithelial wound healing via targeting CDC25A,CARM1,and LASP1

作     者：Qiongjie Cao Weiwei Xu Weiwei Chen Dewei Peng Qi Liu Jing Dong Peter S.Reinach Dongsheng Yan 

作者机构：School of Ophthalmology and OptometryEye HospitalWenzhou Medical University270 Xueyuan RoadWenzhou 325027ZhejiangChina State Key Laboratory of OphthalmologyOptometry and Visual ScienceWenzhouZhejiangChina 

出 版 物：《Eye and Vision》 (眼视光学杂志（英文）)

年 卷 期：2020年第7卷第2期

页      面：336-346页

核心收录：

学科分类：1002[医学-临床医学] 100212[医学-眼科学] 10[医学] 

基　　金：This work was supported,in part,by the 973 Project(2012CB722303)from the Ministry of Science and Technology of China,Science Foundation of Wenzhou Medical University(QTJ11020) the Science and Technology Project of Wenzhou(Grant No.Y20160188) 

主　　题：miR-184 Corneal epithelial wound healing Proliferation Migration CDC25A CARM1 LASP1 

摘      要：Background:MicroRNAs(miRNAs)play critical roles in corneal development and functional *** previous study identified miR-184 as one of the most highly expressed miRNAs in the corneal *** though its expression level plummeted dramatically during corneal epithelial wound healing(CEWH),its precise role in mediating corneal epithelial renewal was *** present study aimed to reveal the function and mechanism of miR-184 in regulating ***:Quantitative RT-PCR analysis characterized the miR-184 expression pattern during CEWH in *** miR-184 injection determined its effect on this process in *** evaluated the effects of miR-184 and its target genes on the proliferation,cell cycle,and migration of human corneal epithelial cells(HCECs)using MTS,flow cytometry,and wound-healing assay,*** analysis,in conjunction with gene microarray analysis and cell-based luciferase assays,pinpointed gene targets of miR-184 contributing to ***:MiR-184 underwent marked downregulation during mouse *** miR-184 overexpression delayed this process in ***,miR-184 transfection into HCECs significantly inhibited cell proliferation,cell cycle progression,and cell ***-184 directly targeted CDC25A,CARM1,and LASP1,and downregulated their expression in ***1 downregulation inhibited both HCEC proliferation and migration,whereas a decrease in LASP1 gene expression only inhibited ***:Our results demonstrate that miR-184 inhibits corneal epithelial cell proliferation and migration via targeting CDC25A,CARM1,and LASP1,suggesting it acts as a negative modulator during ***,identifying strategies to suppress miR-184 expression levels has the potential to promote CEWH.