Oxidative DNA damage in peripheral leukocytes and its association with expression and polymorphisms of hOGG_1:A study of adolescents in a high risk region for hepatocellular carcinoma in China

作     者：TaoPeng Han-MingShen Zhi-MingLiu Lu-NanYan Min-HaoPeng Le-QunLi Ren-XiangLiang Zong-LiangWei BarryHalliwell ChoonNamOng 

作者机构：DepartmentofHepatobiliarySugeryFirstAffiliatedHospitalofGuangxiMedicalUniversityNanning530021GuangxiZhuangAutonomousRegionChina DepartmentofCommunityOccupationalandFamilyMedicineNationalUniversityofSingaporeSingapore DepartmentofGeneralSurgeryFirstAffiliatedHospitalofWestChinaUniversityofMedicalSciencesChengdu610041SichuanZhuangAutonomousRegionChina FusuiCancerResearchInstituteFusuiCounty532100GuangxiProvinceChina DepartmentofBiochemistryNationalUniversityofSingaporeSingapore 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2003年第9卷第10期

页      面：2186-2193页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：the Guangxi Natural Sciences Grant,No.GKZ9912028 and No.GKJ0236030 Guangxi Educational Committee Grant,No.GZBH 2000-272 Guangxi Health Ministry Medicine Grant,No.Z2001087 Singapore Science Grant,No.R-186-000-044-213 

主　　题：外周血 白血球 DNA氧化损伤 基因多态性 肝细胞癌 青年人 高危因素 

摘      要：AIM: To study the oxidative DNA damage to adolescents of hepatocellular carcinoma (HCC) families in Guangxi Zhuang Autonomous Region, China.METHODS: Peripheral leukocytes DNA 7, 8-dihydro-8-oxoguanine (8-oxoG) and repair enzyme hOGG1 were quantified by flow-cytometry. hOGG1-Cys326Ser single nucleotide polymorphism (SNP) was distinguished by polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) assay.RESULTS: There was a positive correlation between 8-oxoG and repair enzyme hOGG1 expression (P0.001). HCC children (n=21) in Fusui county had a higher level of hOGG1(P0.01) and a lower level of 8-oxoG (P0.05) than the controls (n=63) in Nanning city. Children in Nanning exposed to passive-smoking had a higher hOGG1 expression (P0.05)than the non-exposers. 8-oxoG and hOGG1 were negatively correlated with body mass index, while hOGG1 was positively correlated with age. There was a peak of 8-oxoG level nearby the 12 year point. Individuals with the hOGG1 326Ser allele had a significantly marginal higher concentration of leukocyte 8-oxoG level than hOGG1 326Cys allele.CONCLUSION: This is the first report using flow-cytometry to simultaneously quantify both the DNA oxidative damage and its repairing enzyme hOGG1. The results provide new insights towards a better understanding of the mechanisms of oxidative stress in a population highly susceptible to hepatocarcinogenesis.