Isolation and Identification of Triazophos-degrading Bacillus subtilis str. C-Y106 and Its Degradation Characteristics

作     者：陈勇辉 付桂明 万茵 韩蓓 罗阳帆 王建涛 李红歌 陈建芳 柴建新 CHEN Yong-hui;FU Gui-ming;WAN Yin;HAN Bei;LUO Yang-fan;WANG Jian-tao;LI Hong-ge;CHEN Jian-fang;CHAI Jian-xin

作者机构：南昌大学食品科学与技术国家重点实验室江西南昌330047 南昌大学生命科学与食品工程学院江西南昌330031 南昌大学中德食品工程中心江西南昌330047 西安交通大学医学院公共卫生系陕西西安710061 

出 版 物：《Agricultural Science & Technology》 (农业科学与技术（英文版）)

年 卷 期：2010年第11卷第8期

页      面：77-80,113页

学科分类：07[理学] 09[农学] 0903[农学-农业资源与环境] 0713[理学-生态学] 

主　　题：Triazophos-degrading Bacillus subtilis str.C-Y106 Degradation characteristics 

摘      要：[Objective] The aim was to isolate the triazophos-degrading strain and study its degradation characteristics. [Method] A triazophos-degrading bacterium strain C-Y106 was isolated from sludge in an aeration tank of triazophos manufacture. Then the strain C-Y106 was identified according to the morphology,physiological and biochemical characteristics,and 16S rRNA sequence analysis. The effect of medium with different nutrients on triazophos-degrading rate by C-Y106 was studied. [Result] The strain C-Y106 was identified as Bacillus subtilis. The strain C-Y106 could grow in the mineral salt medium with 40 mg/L of triazophos as the sole sources of carbon,Nitrogen and Phosphorus. The triazophos-degrading rate was the highest as 76.8% in the mineral salt medium with 40 mg/L of triazophos as the sole source of Phosphorus,after being incubated at 31 ℃,pH 8.0 and 150 r/min for 60 h. [Conclusion] The research had provided theoretical basis for the identification and purification of enzymes for triazophos degradation.