Isolation and identification of adipose-derived stromal/stem cells from breast cancer patients after exposure neoadjuvant chemotherapy
作者机构：Department of SurgeryCooper University HospitalCamdenNJ 08103United States Cooper Medical School of Rowan UniversityCamdenNJ 08103United States
出 版 物：《World Journal of Experimental Medicine》 (世界实验医学杂志)
年 卷 期：2020年第10卷第3期
页 面：26-40页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：Human adipose-derived stromal/stem cells Breast cancer Neoadjuvant chemotherapy Chemotherapeutic agents Adipogenic differentiation Proliferation
摘 要：BACKGROUND With recent research advances,adipose-derived stromal/stem cells(ASCs)have been demonstrated to facilitate the survival of fat grafts and thus are increasingly used for reconstructive procedures following surgery for breast ***,in patients,following radiation and chemotherapy for breast cancer suggest that these cancer treatment therapies may limit stem cell cellular functions important for soft tissue wound *** clinical translation to patients that have undergone cancer treatment,it is necessary to understand the effects of these therapies on the ASC s ability to improve fat graft survival in clinical *** To investigate whether the impact on ASCs function capacity and recovery in cancer patients may be due to the *** ASCs were isolated from the cancerous side and noncancerous side of the breast from the same patients with receiving neoadjuvant chemotherapy(NAC)or notreceiving *** were in vitro treated with 5-fluorouracil(5-FU),doxorubicin(DXR),and cyclophosphamide(Cytoxan)at various *** stem cells yield,cell viability,and proliferation rates were measured by growth curves and MTT *** capacity for adipogenesis was determined by qPCR analysis of the specific gene markers and histological *** No significant differences were observed between the yield of ASCs in patients receiving NAC treatment and not-receiving *** yield from the cancerous side of the breast showed lower than the noncancerous side of the breast in both patients receiving NAC and not-receiving *** proliferation rates of ASCs from patients didn’t differ much before and after NAC upon in vitro culture,and these cells appeared to retain the capacity to acquire adipocyte traits simile to the ASCs from patients not-receiving *** cessation and washout of the drugs for another a week of culturing,ASCs showed a slow recovery of cell growth capacity in 5-FU-treated groups but was not
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