Novel Primate Bocaparvovirus Species 3 Identified in Wild Macaca Mulatta in China

作     者：Yuanyun Ao Zhaojun Duan Yuanyun Ao;Zhaojun Duan

作者机构：Chinese Center for Disease Control and PreventionNational Institute for Viral Disease Control and PreventionBeijingChina 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2020年第35卷第1期

页      面：34-42页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 071005[理学-微生物学] 10[医学] 

基　　金：the National Science and Technology Major Project(2018ZX10301408-001) 

主　　题：Primate bocaparvovirus(BOV) Novel species Macaca mulatta Sequence analysis Phylogenetic analysis 

摘      要：Primate bocaparvovirus(BOV) is a possible cause of respiratory disorders and gastroenteritis in humans. However, the diversity and evolution of these viruses remain largely unknown, despite the identification of a growing number of BOVs in non-human primates(NHPs). Here, we report the identification of a novel BOV(provisionally named Macaca mulatta bocaparvovirus [MmBOV]) in the feces of wild Macaca mulatta in China by viral metagenomic analysis. Seven of 400 fecal samples from Macaca mulatta were positive for MmBOV. An almost complete genome sequence of 4,831 nucleotides was obtained, which had genomic organization and protein motifs similar to human bocaviruses(HOBVs), and shared characteristically low G/C content and weak codon usage bias. Sequence analyses of NS1, NP1, and VP1 revealed that Mm BOV was most closely related to HBOV4 of Primate bocaparvovirus 2(approximately 68.4%/70.6%, 73.3%/67.6%, and 70.4%/73.1% nucleotide/amino acid identities, respectively). Additionally, phylogenetic analysis revealed that Mm BOV formed an independent peripheral branch, but clustered closely with those of the Primate bocaparvovirus species in the BOV genus(particularly HBOV4). These data strongly suggest that HBOV4 originated from NHP bocaparvoviruses around 200–300 years ago, and that NHPs may act as HBOV reservoirs. Following the International Committee of Taxonomy for Viruses guidelines, we propose Mm BOV as a new species(tentatively named Primate bocaparvovirus 3) in the genus Bocaparvovirus, which is the first report of a novel species of primate BOV. Our data facilitate future research on the genetic diversity and evolution of primate bocaparvoviruses and highlight the importance of bocaparvovirus surveys in wild NHPs.