GC-MS法测定Calophyllum inophyllum L.中的红厚壳素(英文)

作     者：Ha Minh Hien Paul WS Heng Nguyen Van Thi Nguyen Khac Quynh Cu Vo Thi Bach Hue 

作者机构：Institute of Drug Quality Control-Ho Chi Minh CityHo Chi Minh CityVietnam Department of PharmacyFaculty of ScienceGEA-NUS Pharmaceutical Processing Research LaboratoryNational University of Sin-gaporeSingapore Faculty of PharmacyUniversity of Medicine and PharmacyHo Chi Minh CityVietnam 

出 版 物：《中国天然药物》 (Chinese Journal of Natural Medicines)

年 卷 期：2011年第9卷第6期

页      面：429-434页

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100704[医学-药物分析学] 1005[医学-中医学] 1002[医学-临床医学] 0703[理学-化学] 10[医学] 

基　　金：supported by the financial support from the Nagai Foundation Tokyo 

主　　题：Calophyllum inophyllum Calophyllolide GC-MS Validation 

摘      要：AIM:To establish a GC-MS method for the determination of calophyllolide in Calophyllum inophyllum *** its application in quality control of natural ***:Methanolic extract from Calophyllum inophyllum *** *** was isolated,purified and then identified to serve as a chemical marker for development and validation a GC-MS method according to ICH guideline and *** established method was proved to be useful in quality control with the aid of Principle Component Analysis (PCA),a multivariate data analysis ***:The proposed method was selective against mass spectral deconvoluting software *** linear range was from 3.125-50 μg·*** RSD of intra-and inter-day precision variations were less than 3% and the mean recovery was (101.22±1.98)% (RSD=1.95%).PCA was used to indicate a differentiation of batches in production as well as proving that calophyllolide has influence on the discrimination of extracts from different ***:The proposed capillary gas chromatography/mass spectrometric method for the determination of calophyllolide obtained from resin of Calophyllum inophyllum *** oil showed its suitability,simplicity,rapidity and precision,and it can be used for chemical *** is the first time such a technique was reported in *** multivariate data analysis technique was employed to provide an extensive but inclusive method for quality control of the extracts,to ensure consistency for batch to batch of extracts to be used for product development.