CTP synthase forms cytoophidia in archaea

作     者：Shuang Zhou Hua Xiang Ji-Long Liu Shuang Zhou;Hua Xiang;Ji-Long Liu

作者机构：School of Life Science and TechnologyShanghaiTech UniversityShanghai201210China University of Chinese Academy of SciencesBeijing100049China Institute of Biochemistry and Cell BiologyShanghai Institutes for Biological SciencesChinese Academy of SciencesShanghai200031China State Key Laboratory of Microbial ResourcesInstitute of MicrobiologyChinese Academy of SciencesBeijing100101China 

出 版 物：《Journal of Genetics and Genomics》 (遗传学报（英文版）)

年 卷 期：2020年第47卷第4期

页      面：213-223页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 

基　　金：supported by ShanghaiTech University and National Natural Science Foundation of China(No.31771490) 

主　　题：Archaea CTP synthase Cytoophidium Haloarcula hispanica 

摘      要：CTP synthase(CTPS)is an important metabolic enzyme that catalyzes the rate-Iimiting reaction of nucleotide CrP de novo *** 2010,a series of studies have demonstrated that CTPS can form filamentous structures in bacteria and eukaryotes,which are termed ***,it is unknown whether cytoophidia exist in the third domain of life,*** Haloarcula hispanica as a model system,here we demonstrate that CTPS forms distinct intracellular compartments in *** stimulated emission depletion microscopy,we find that the structures of *** CTPS are elongated,similar to cytoophidia in bacteria and *** Haloarcula cells are cultured in lowsalt medium,the occurrence of cytoophidia increases *** addition,treatment of *** with a glutamine analog or overexpression of CTPS can promote cytoophidium *** study reveals that CTPS can fo rm cytoophidia in all three domains of life,suggesting that forming cytoophidia is an ancient property of CTPS.