Microbial remediation of a pentachloronitrobenzene-contaminated soil under Panax notoginseng: A field experiment

作     者：Ning ZHANG Dong GUO Ye ZHU Xiaomi WANG Lingjia ZHU Fang LIU Ying TENG Peter CHRISTIE Zhengao LI Yongming LUO Ning ZHANG;Dong GUO;Ye ZHU;Xiaomi WANG;Lingjia ZHU;Fang LIU;Ying TENG;Peter CHRISTIE;Zhengao LI;Yongming LUO

作者机构：College of Resource and Environmental EngineeringGuizhou UniversityGuiyang 550025(China) Key Laboratory of Soil Environment and Pollution RemediationInstitute of Soil ScienceChinese Academy of SciencesNanjing 210008(China) Infinitus(China)Co.LTDJiangmen 529156(China) Institute of Coastal Zone ResearchChinese Academy of SciencesYantai 264003(China) 

出 版 物：《Pedosphere》 (土壤圈（英文版）)

年 卷 期：2020年第30卷第4期

页      面：563-569页

核心收录：

学科分类：12[管理学] 1204[管理学-公共管理] 082803[工学-农业生物环境与能源工程] 08[工学] 0828[工学-农业工程] 0818[工学-地质资源与地质工程] 0903[农学-农业资源与环境] 0901[农学-作物学] 120405[管理学-土地资源管理] 

基　　金：This research was funded by grants from the Major Science and Technique Programs of Yunnan Province,China(No.2016ZF001) the Science and Technique Programs of Yunnan Province,China(No.2017IB038) 

主　　题：Cupriavidus sp.YNS-85 enzyme activities in-situ remediation PCNB removal soil 

摘      要：Pentachloronitrobenzene(PCNB)is an organochlorine fungicide that is mainly used in the prevention and control of diseases in crop *** removal is used as a promising method for in-situ removal of many organic pesticides and pesticide residues.A short-term field experiment(1 year)was conducted to explore the potential role of a PCNB-degrading bacterial isolate,Cupriavidus ***-85,in the remediation of a PCNB-contaminated soil on which Panax notoginseng was *** following three treatments were used:i)control soil amended with wheat bran but without YNS-85,ii)soil with 0.15 kg m-2of solid bacterial inoculum(A),and iii)soil with 0.30 kg m-2of solid bacterial inoculum(B).The removal of soil PCNB during the microbial remediation was monitored using gas *** catalase and fluorescein diacetate(FDA)esterase activities were determined using *** addition,cultivable bacteria,fungi,and actinomycetes were counted by plating serial dilutions,and the microbial biodiversity of the soil was analyzed using *** 1 year of in-situ remediation,the soil PCNB concentrations decreased significantly by 50.3%and 74.2%in treatments A and B,respectively,when compared with the uninoculated *** soil catalase activity decreased in the presence of the bacterial isolate,the FDA esterase activity decreased in treatment A,but increased in treatment *** significant changes in plant biomass,diversity of the soil microbial community,or physicochemical properties of the soil were observed between the control and inoculated groups(P0.05).The results indicate that Cupriavidus ***-85 is a potential candidate for the remediation of PCNB-contaminated soils under ***.