提高MALDI-MS测量溶菌酶分子量灵敏度的研究

作     者：石磊 王勇 刘志强 刘淑莹 

作者机构：中国科学院长春应用化学研究所 

出 版 物：《高等学校化学学报》 (Chemical Journal of Chinese Universities)

年 卷 期：2002年第23卷第12期

页      面：2247-2249页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 0703[理学-化学] 

主　　题：MALDI-MS 测量 分子量 灵敏度 基质辅助激光解吸电离质谱 溶菌酶 硝酸纤维素膜 

摘      要：Sodium dodecyl sulfate(SDS) is a powerful solubilizing detergent which is often used during the separation of highly complex protein mixtures by one- or two-dimensional(2D) gel electrophoresis. Matrix-assisted laser desorption/ionization mass spectrometry(MALDI-MS) is a widely used technique for mass spectrometric analysis of some protein molecules compared to other techniques. But the presence of SDS or some salts usually leads to signal deterioration when using MALDI-MS. A method for using nitrocellulose membrane as the solid-phase carrier combined with n-octyl-β-D-glucopyranoside in the matrix highly enhances the sensitivity of the molecular mass determination of lysozyme. This technique has the advantage that the signal-to-noise of the molecular weight profile is improved compared with the mass spectrum and the profile is relatively easy to interpret.