Analysis on the Origin and Phylogenetic Status of Tong Sheep Using 12 Blood Protein and Nonprotein Markers

作     者：孙伟 常洪 杨章平 耿荣庆 角田健司 任战军 陈宏宇 Musa H.Hussein Wei Sun;Hong Chang;Zhangping Yang;Rongqing Geng;Kenji Tsunoda;Zhanjun Ren;Hongyu Chen;Musa H. Hussein

作者机构：扬州大学动物科学与技术学院扬州225009 日本昭和大学医学部 西北农林科技大学杨陵712100 

出 版 物：《Journal of Genetics and Genomics》 (遗传学报（英文版）)

年 卷 期：2007年第34卷第12期

页      面：1097-1105页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071002[理学-动物学] 

基　　金：the International Cooperation Item of the National Natural Science Foundation of China (No. 30213009, 30310103007, 30410103150) Natural Science Foundation of Jiangsu Province of China (No. BK2007556) Basic Natura Science Foundation for Colleges and Universities Jiangsu Province (No. NK051039) the New Century Talent Project of Yangzhou University in China 

主　　题：Tong sheep origin phylogenetic status genetic approach degree phylogenetic relationship clustering 

摘      要：This study is based on the Tong sheep obtained by the random sampling method of typical colonies in the central area of Baishui County in Shaanxi Province, China. An investigation was undertaken to clarify the gene constitution of blood protein and nonprotein types of Tong sheep. Twelve genetic markers were examined by starch-gel electrophoresis and cellulose acetate electrophoresis. Polymorphism in Tong sheep was found at the following 10 loci, transferrin （Tf）, alkaline phosphatase （Alp）, leucine aminopeptidase （Lap）, arylesterase （Ary-Es）, hemoglobin-β （Hb-β）, X-protein （X-p）, carbonic anhydrase （CA）, catalase （Cat）, malate dehydrogenase （MDH）, and lysine （Ly）, whereas, albumin （A1） and postalbumin （Po） loci were monomorphic. Genetic approach degree method and phylogenetic relationship clustering method were used to judge the origin and phylogenetic status of Tong sheep. Results from both methods maintained that Tong sheep belonged to the ＂Mongolia group＂, and Mongolia sheep was the origin of Tong sheep. This was also supported by the history of Tong sheep breeding. Compared to the phylogenetic relationship clustering method, the genetic approach degree method was more reliable for the extraction from East and South of Central Asia, and was more effective in reflecting the breeding course of Tong sheep.