银杏胚轴、子叶诱导胚状体发生及成苗的研究

作     者：郭长禄 陈力耕 何新华 Syed Asgar Tori 袁海英 Guo Changlu;Chen Ligeng;He Xinhua;Syed Asgar Tori;Yuan Haiying

作者机构：浙江大学农业部园艺植物生长发育与生物技术重点开放实验室 

出 版 物：《林业科学》 (Scientia Silvae Sinicae)

年 卷 期：2005年第41卷第2期

页      面：178-181,F003页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 09[农学] 0902[农学-园艺学] 090201[农学-果树学] 

基　　金：国家自然科学基金资助项目 ( 3 0 0 70 63 4) 

主　　题：胚状体发生 胚轴 银杏 成苗 诱导 子叶 axis 6-BA best and was for NAA the mg The pla 

摘      要：Embryonic axis and cotyledon of immature and mature embryos were induced as explants for embryogenesis in Ginkgo biloba. Results showed somatic embryos could be induced only through embryonic axis and cotyledon of immature embryos. MK + NAA 1 mg·L -1 + 6_BA 1 mg·L -1 was the best medium for somatic embryogenesis from embryonic axis, and somatic embryogenesis rate was 45.28%. MK + NAA 1.5 mg·L -1 + 6_BA 1 mg·L -1 was the best medium for somatic embryogenesis from cotyledon, and somatic embryogenesis rate was 12.9%. The growth and development of somatic embryos were promoted by adding 10% coconut milk into MK, and 34.48% somatic embryos could develop into plants.