Isolation, Identification of Differentially Expressed Sequence Tags in the Backfat Tissue from Meishan, Large White and MeishanLarge White Cross Pigs

作     者：LIUYong-gang XIONGYuan-zhu DENGChang-yan 

作者机构：KeyLaboratoryofSwineGeneticsandBreeding／MinistryofAgricultureHuazhongAgriculturalUniversityWuhan430070P.R.China 

出 版 物：《Agricultural Sciences in China》 (中国农业科学（英文版）)

年 卷 期：2005年第4卷第1期

页      面：54-58页

学科分类：0905[农学-畜牧学] 09[农学] 090501[农学-动物遗传育种与繁殖] 

基　　金：国家重点基础研究发展计划(973计划)(G2000016105) 

主　　题：Pig mRNA differential display Semi-quantitative RT-PCR Expressed sequence tag 

摘      要：In order to detect the molecular mechanism of heterosis in pigs, the mRNA differential display technique was performedto investigate the differences of gene expression in the backfat tissue from Meishan, Large White and MeishanLargeWhite cross pigs. Nine 3 -end anchored primers in combination with ten 5 -end arbitrary primers were used to perform thedifferential display PCR and nearly 3 000 reproducible bands were examined. Fifteen expressed sequence tags that weredifferentially expressed were isolated and then identified through semi-quantitative RT-PCR. BLAST analysis revealedthat the fifteen expressed sequence tags (ESTs) were not homologous to any of the known porcine genes or ESTs. Thesenovel ESTs were then submitted to GenBank.