Cadmium isotope fractionation reveals genetic variation in Cd uptake and translocation by Theobroma cacao and role of natural resistanceassociated macrophage protein 5 and heavy metal ATPase-family transporters
作者机构：Department of Earth Science and EngineeringImperial CollegeLondon SW72BPUK School of AgriculturePolicy and DevelopmentUniversity of ReadingReading RG66BZUK School of EnvironmentEarth and Ecosystem SciencesThe Open UniversityMilton Keynes MK76AAUK Imaging and Analysis CentreThe Natural History MuseumLondon SW75BDUK Present address:Department of Plant BiologyInstitute of BiologyUniversity of CampinasCampinasSao Paulo 13083-970Brazil Present address:National Measurement LaboratoryLGCQueen’s RoadTeddington TW110LYUK
出 版 物：《Horticulture Research》 (园艺研究（英文）)
年 卷 期：2020年第7卷第1期
页 面：2006-2016页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种]
基 金：supported by grants from the GCRF programmes of STFC(grants ST/P003257/1 4070200262)and UKRI(grant EP/S515875/1)to M.R.as well as funding from Mars Wrigley Confectionery to J.M.D.and M.R.and Cocoa Research UK to J.M.D.V.H.d.O.was supported by CAPES(Coordination for the Improvement of Higher Education Personnel—Brazil)[project:13462-13-0]
主 题：roots transformed signature
摘 要：In response to new European Union regulations,studies are underway to mitigate accumulation of toxic cadmium(Cd)in cacao(Theobroma cacao,Tc).This study advances such research with Cd isotope analyses of 19 genetically diverse cacao clones and yeast transformed to express cacao natural resistance-associated macrophage protein(NRAMP5)and heavy metal ATPases(HMAs).The plants were enriched in light Cd isotopes relative to the hydroponic solution withΔ^(114/110)Cd_(tot-sol)=−0.22±0.08‰.Leaves show a systematic enrichment of isotopically heavy Cd relative to total plants,in accord with closed-system isotope fractionation ofΔ^(114/110)C_(dseq-mob)=−0.13‰,by sequestering isotopically light Cd in roots/stems and mobilisation of remaining Cd to *** findings demonstrate that(i)transfer of Cd between roots and leaves is primarily unidirectional;(ii)different clones utilise similar pathways for Cd sequestration,which differ from those of other studied plants;(iii)clones differ in their efficiency of Cd *** yeast that expresses TcNRAMP5(*** natural resistance-associated macrophage gene)had isotopically lighter Cd than did *** suggests that NRAMP5 transporters constitute an important pathway for uptake of Cd by *** isotope signatures of transgenic yeast expressing HMA-family proteins suggest that they may contribute to Cd *** data are the first to record isotope fractionation induced by transporter proteins in vivo.
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