Involvement of moesin phosphorylation in ischemia/reperfusion induced inner blood-retinal barrier dysfunction

作     者：Jing Xu Qiong Liu Ming Ma Lin-Jiang Chen Jian Yu Ke Xiong Jing Wu 

作者机构：Department of OphthalmologyNanfang HospitalSouthern Medical UniversityGuangzhou 510515Guangdong ProvinceChina Huiqiao Medical CenterNanfang HospitalSouthern Medical UniversityGuangzhou 510515Guangdong ProvinceChina 

出 版 物：《International Journal of Ophthalmology(English edition)》 (国际眼科杂志（英文版）)

年 卷 期：2020年第13卷第4期

页      面：545-551页

核心收录：

学科分类：1002[医学-临床医学] 100212[医学-眼科学] 10[医学] 

基　　金：Supported by the Science and Technology Planning Project of Guangdong Province(No.201607010386) the Science and Technology Planning Project of Guangzhou(No.201504290959196) 

主　　题：retinal ischemia-reperfusion moesin p38 mitogen-activated protein kinase inner blood-retinal barrier mice 

摘      要：AIM: To investigate the role of moesin and its underlying signal transduction in retinal vascular damage induced by retinal ischemia-reperfusion(RIR) ***: C57 BL/6 mice were subjected to continued ischemia for 45 min, followed by blood reperfusion. The expression and phosphorylation of moesin in retinal vessels were detected by immunohistochemistry and Western blotting. The inner blood-retinal barrier was evaluated using FITCdextran leakage assay on whole-mount retina. Further studies were conducted to explore the effects of p38 mitogen-activated protein kinase(MAPK) pathway on the involvement of moesin in RIR-evoked retinal vascular hyperpermeability response. RESULTS: It revealed that RIR induced moesin phosphorylation in a time-dependent manner after reperfusion. The phosphorylation of moesin was alleviated by inhibitions of p38 MAPK, while this treatment also ameliorated the dysfunction of inner blood-retinal barrier. CONCLUSION: The results suggest that moesin is involved in RIR-evoked retinal vascular endothelial dysfunction and the phosphorylation of moesin is triggered via p38 MAPK activation.