FKF1 F-box protein promotes flowering in part by negatively regulating DELLA protein stability under long-day photoperiod in Arabidopsis^FA

作     者：Jindong Yan Xinmei Li Bingjie Zeng Ming Zhong Jiaxin Yang Piao Yang Xin Li Chongsheng He Jianzhong Lin Xuanming Liu Xiaoying Zhao Jindong Yan;Xinmei Li;Bingjie Zeng;Ming Zhong;Jiaxin Yang;Piao Yang;Xin Li;Chongsheng He;Jianzhong Lin;Xuanming Liu;Xiaoying Zhao

作者机构：College of BiologyHunan Province Key Laboratory of Plant Functional Genomics and Developmental RegulationState Key Laboratory of Chemo/Biosensing and ChemometricsHunan UniversityChangsha 410082China Shenzhen InstituteHunan UniversityShenzhen 518057China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2020年第62卷第11期

页      面：1717-1740页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：We thank Dr.Hongtao Liu for her kind advice on thiswork,Dr.Chentao Lin for providing fkf1-1 seeds,Dr.Taiping Sun for providing ga1-3(Col background),Dr.Xingwang Deng for 35S:TAP-RGA transgenic seedsand della mutant seeds,Dr.Xiangdong Fu for gaiseeds,Dr.Suiwen Hou for rga-28 seeds,Dr.DaoxinXie for ga1-3(Ler background),Q1,Q2,and pentamutant seeds.This work was supported by the Na-tional Natural Science Foundation of China(No.31171176) Natural Science Foundation of HunanProvince(No.2018JJ3036) the Basic Research Pro-gram of Shenzhen Municipal Science and Technologylnnovation Committee(No.JCYJ20170818112212721) and the Basic Research Program of ChangshaMunicipal Science and Technology(No.kq1901o28) 

主　　题：expression. photoperiod stability 

摘      要：FLAVIN-BINDING KELCH REPEAT F-BOX 1(FKF1)encodes an F-box protein that regulates photoperiod flowering in Arabidopsis under long-day conditions(LDs).Gibberellin(GA)is also important for regulating flowering under ***,how FKF1 and the GA pathway work in concert in regulating flowering is not fully ***,we showed that the mutation of FKF1 could cause accumulation of DELLA proteins,which are crucial repressors in GA signaling pathway,thereby reducing plant sensitivity to GA in *** in vitro and in vivo biochemical analyses demonstrated that FKF1 directly interacted with DELLA ***,we showed that FKF1 promoted ubiquitination and degradation of DELLA *** of genetic data revealed that FKF1 acted partially through DELLAs to regulate flowering under *** addition,DELLAs exerted a negative feedback on FKF1 ***,these findings demonstrate that FKF1 promotes flowering partially by negatively regulating DELLA protein stability under LDs,and suggesting a potential mechanism linking the FKF1 to the GA signaling DELLA proteins.