角膜内皮炎患者的房水中发现巨细胞病毒

作     者：Koizumi N. Yamasaki K. Kawasaki S. 安胜(译) 

作者机构：不详 Department of Ophthalmology Kyoto Prefectural University of Medicine Kawaramachi Hirokoji Kamigyoku Kyoto 602-0841 Japan 

出 版 物：《世界核心医学期刊文摘（眼科学分册）》 (Digest of the World Core Medical Journals:Ophthalmology)

年 卷 期：2006年第2卷第6期

页      面：11-12页

学科分类：1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100212[医学-眼科学] 100103[医学-病原生物学] 10[医学] 

主　　题：巨细胞病毒(CMV) 角膜内皮炎 男性患者 房水 水痘带状疱疹病毒 Southern 单纯疱疹病毒 聚合酶链反应 内皮细胞损害 DNA 

摘      要：PURPOSE: To report cytomegalovirus (CMV) DNA in aqueous humor from a patient with unilateral corneal endotheliitis. DESIGN: Case report. METHODS: A 51-year-old man presented with unilateral corneal endotheliitis with linear keratic precipitates and coin-shaped lesions. Tear and aqueous humor samples were subjected to polymerase chain reaction to look for DNA from herpes simplex virus (HSV),varicella zoster virus (VZV),and CMV. RESULTS: Aqueous humor from the diseased eye contained DNA from CMV but not HSV or VZV. Its specificity was confirmed by Southern blot tests. Intravenous ganciclovir treatment resulted in the localization of his corneal edema and the reduction in keratic precipitates. There was severe destruction of corneal endothelial cells. CMV DNA was not detected in tears or control samples. CONCLUSIONS: In this healthy man with corneal endotheliitis,we detected CMV DNA in aqueous humor from the affected eye,but not HSV or VZV. This suggests that CMV may cause corneal endotheliitis in patients without immunodeficiency.