Acute effects of human protein S administration after traumatic brain injury in mice

作     者：Xiaowei Wang Jing Tong Xiaodi Han Xiaoming Qi Jun Zhang Erxi Wu Jason H.Huang 

作者机构：Center for Translational NeuromedicineUniversity of RochesterRochesterNYUSA Department of Neurosurgery4th Affiliated Hospital of Hebei Medical UniversityShijiazhuangHebei ProvinceChina Department of NeurosurgeryTiantan HospitalBeijingChina Department of NeurosurgeryBaylor Scott&White HealthTempleTXUSA Department of NeurosurgeryPLA General HospitalBeijingChina College of MedicineTexas A&M Health Science CenterTempleTXUSA 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2020年第15卷第11期

页      面：2073-2081页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：supported in part by a University of Rochester Institutional Grant(2011NSG-Huang,to JHH) National Institute of Health(NIH-R01-NS-067435,to JHH) the Hellen Vosberg McCrillus Plummer and Robert Edward Lee Plummer,Jr,Endowment fund from Baylor Scott&White Medical Center(to JHH) 

主　　题：apoptosis aquaporin-4 controlled cortical impact edema inflammation protein S TBI therapy traumatic brain injury 

摘      要：Despite years of effort,no effective acute phase treatment has been discovered for traumatic brain *** impediment to successful drug development is entangled secondary injury *** we show that protein S,a natural multifunctional protein that regulates coagulation,inflammation,and apoptosis,is able to reduce the extent of multiple secondary injuries in traumatic brain injury,and therefore improve *** subjected to controlled cortical impact were treated acutely(10–15 minutes post-injury)with a single dose of either protein S(1 mg/kg)or vehicle phosphate buffered saline via intravenous *** 24 hours post-injury,compared to the non-treated group,the protein S treated group showed substantial improvement of edema and fine motor coordination,as well as mitigation of progressive tissue *** and western blot targeting caspase-3,B-cell lymphoma 2(Bcl-2)along with terminal deoxynucleotidyl transferase dUTP nick end labeling(TUNEL)assay revealed that apoptosis was suppressed in treated *** targeting CD11 b showed limited leukocyte infiltration in the protein S-treated ***,protein S treatment increased the ipsilesional expression of aquaporin-4,which may be the underlying mechanism of its function in reducing *** results indicate that immediate intravenous protein S treatment after controlled cortical impact is beneficial to traumatic brain injury *** Use Protocols(AUPs)were approved by the University Committee on Animal Resources(UCAR)of University of Rochester Medical Center(approval ***-2008-102 R)on November 12,2013.