Human β-defensin 3 gene modification promotes the osteogenic differentiation of human periodontal ligament cells and bone repair in periodontitis

作     者：Lingjun Li Han Jiang Rixin Chen Jing Zhou Yin Xiao Yangheng Zhang Fuhua Yan Lingjun Li;Han Jiang;Rixin Chen;Jing Zhou;Yin Xiao;Yangheng Zhang;Fuhua Yan

作者机构：Nanjing Stomatological HospitalMedical School of Nanjing UniversityNanjingChina People’s Hospital of Suzhou National New&Hi-Tech Industrial Development ZoneSuzhouChina The Affiliated Stomatological HospitalZhejiang University School of MedicineKey Laboratory of Oral Biomedical Research of Zhejiang ProvinceZhejiang University School of StomatologyHangzhouChina Institute of Health and Biomedical Innovation&the Australia-China Centre for Tissue Engineering and Regenerative MedicineQueensland University of TechnologyBrisbaneAustralia 

出 版 物：《International Journal of Oral Science》 (国际口腔科学杂志（英文版）)

年 卷 期：2020年第12卷第2期

页      面：162-171页

核心收录：

学科分类：1003[医学-口腔医学] 100302[医学-口腔临床医学] 10[医学] 

基　　金：supported by the National Natural Science Foundation Project(No.81771078 and No.81570982) Jiangsu Provincial Medical Innovation Team(No.CXTDB2017014) the Nanjing Clinical Research Center for Oral Diseases(No.2019060009) 

主　　题：period periodontal ligament 

摘      要：Efforts to control inflammation and achieve better tissue repair in the treatment of periodontitis have been ongoing for ***β-defensin 3,a broad-spectrum antimicrobial peptide has been proven to have a variety of biological functions in periodontitis;however,relatively few reports have addressed the effects of human periodontal ligament cells(h PDLCs)on osteogenic *** this study,we evaluated the osteogenic effects of h PDLCs with an adenoviral vector encoding humanβ-defensin 3 in an inflammatory *** humanβ-defensin 3 gene-modified rat periodontal ligament cells were transplanted into rats with experimental periodontitis to observe their effects on periodontal bone *** found that the humanβ-defensin 3 gene-modified h PDLCs presented with high levels of osteogenesis-related gene expression and calcium ***,the p38 MAPK pathway was activated in this *** vivo,humanβ-defensin 3 gene-transfected rat PDLCs promoted bone repair in SD rats with periodontitis,and the p38 mitogen-activated protein kinase(MAPK)pathway might also have been *** findings demonstrate that humanβ-defensin 3 accelerates osteogenesis and that humanβ-defensin 3 gene modification may offer a potential approach to promote bone repair in patients with periodontitis.