Inhibiting effect of melittin on pathogens of crops

作     者：PAN LingZi NA Jie XING Zhuo FANG HongJun WANG GuanLin 

作者机构：College of Life Science Liaoning Normal University Dalian 116029 China 

出 版 物：《Chinese Science Bulletin》 (CHINESE SCIENCE BULLETIN)

年 卷 期：2007年第52卷第5期

页      面：639-644页

核心收录：

学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 090403[农学-农药学(可授农学、理学学位）] 08[工学] 09[农学] 0904[农学-植物保护] 

基　　金：Supported by the National Natural Science Foundation of China (Grant No. 30170112) the International Cooperation Project (Grant No. 30310103130) 

主　　题：蜂毒肽 作物 病原菌 抑制作用 抑菌机制 抗菌谱 

摘      要：The spectrum of antimicrobial effects of melittin were investigated on 19 pathogens by using a cylin-der-plate method with serial dilutions. Bacteriostatic efficiency and possible mechanistic effects were monitored via growth curves. The mechanism of inhibition was further analyzed by SDS-PAGE, flow cytometry and electron microscopy. Melittin had a wide inhibition spectrum and killed pathogens ef-fectively, and bacteriostatic action was influenced by factors such as pH and temperature. We eluci-dated three inhibitory mechanisms: melittin integrated with the cell membrane causing cell bursting and cytoplasm release, inhibited the synthesis of proteins and caused the cytoplasm to condense, and delayed pathogens in phase I (or phase G1) so that they could not complete the cell cycle. These re-sults suggest that melittin could serve as a broad-spectrum biological pesticide with fast-action and high-efficiency.