Pear pomace water extract reduces adiposity in vivo and in vitro by activating the AMPK-dependent pathway
作者机构：Department of Nutrition and Health SciencesUniversity of NebraskaLincolnNEUSA Department of Food and NutritionHanyang UniversitySeoulRepublic of Korea Department of Food and NutritionMokpo National UniversityJeollanam-doRepublic of Korea
出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)
年 卷 期：2020年第10卷第5期
页 面：208-215页
核心收录：
学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 100403[医学-营养与食品卫生学] 10[医学]
基 金：supported by the Bio-Industry Technology Development Program Ministry of Agriculture Food and Rural Affairs(313019-09-3-HD030)
主 题：Pear pomace AMPK Compound C Obesity Insulin resistance
摘 要：Objective: To explore the inhibitory effect of water extract from pear pomace on abdominal fat accumulation and its underlying mechanism in high fat diet-fed animals. Methods: Three groups of male C57 BL/6 J mice were fed with a 60% kcal fat diet for 8 weeks. Pear pomace water extract(200 or 400 mg/kg body weight) was administered once daily via oral gavage. To confirm the possibility of the water extract of pear pomace acting as an activator of adenosine 5’-monophosphateactivated protein kinase(AMPK), differentiation of 3 T3-L1 preadipocytes was induced in the presence of the water extract of pear pomace with or without compound C. Body weight, food efficacy ratio, insulin resistance, and adipogenic protein expression were measured. Moreover, in the 3 T3-L1 cells, lipid content and lipogenesis-related proteins were measured using Oil Red O staining and Western blotting ***: Body weight gain and total abdominal fat weight were reduced in mice treated with pear pomace water extract. Pear pomace water extract reduced fasting blood glucose and insulin, thereby reducing the homeostatic model assessment of insulin resistance. It also resulted in dose-dependent decreases in triglyceride, total cholesterol, and low-density lipoproteincholesterol. The protein expression of p-AMPK increased, while the expression of AMPK-downstream proteins including PPAR-γ, C/EBPα, SREBP-1 c, ACC, and FAS decreased in the adipose tissue of mice treated with pear pomace water extract. Furthermore, the inhibition of AMPK by compound C blocked pear pomace water extract-induced reduction of lipid content and the expression of lipogenesis-related genes. Conclusions: Pear pomace water extract prevents fat accumulation both in vivo and in vitro by activating AMPK.
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