Presence and Antimicrobial Susceptibility of RE-cmeABC-Positive Campylobacter Isolated from Food-Producing Animals, 2014–2016

作     者：Dejun Liu Weiwen Liu Xing Li Hong Yao Zhangqi Shen Yang Wang Jianzhong Shen Dejun Liu;Weiwen Liu;Xing Li;Hong Yao;Zhangqi Shen;Yang Wang;Jianzhong Shen

作者机构：Beijing Advanced Innovation Center for Food Nutrition and Human HealthCollege of Veterinary MedicineChina Agricultural UniversityBeijing 100193China 

出 版 物：《Engineering》 (工程（英文）)

年 卷 期：2020年第6卷第1期

页      面：34-39页

核心收录：

学科分类：0810[工学-信息与通信工程] 02[经济学] 0202[经济学-应用经济学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 0808[工学-电气工程] 0817[工学-化学工程与技术] 0807[工学-动力工程及工程热物理] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 0812[工学-计算机科学与技术（可授工学、理学学位）] 

基　　金：National Natural Science Foundation of China, NSFC, (31802247) China Postdoctoral Science Foundation, (2018M631638) National Key Research and Development Program of China, NKRDPC, (2017YFC1601501) 

主　　题：RE-cmeABC Campylobacter Ciprofloxacin Florfenicol 

摘      要：Campylobacter spp. are the leading cause of human gastroenteritis worldwide. RE-CmeABC is a newly identified resistance-enhancing multidrug efflux pump of Campylobacter spp. (C. spp.) that confers high-level resistance to fluoroquinolones, phenicols, macrolides, and tetracyclines (TETs), all of which are critical drugs in both human and veterinary medicine. In this study, we analyzed the presence and antimicrobial susceptibility of RE-cmeABC-positive Campylobacter isolates of food-animal origin from three representative regions (Shandong, Shanghai, and Guangdong) in China over three successive years, from 2014 to 2016. A total of 1088 Campylobacter isolates (931 C. coli and 157 C. jejuni) were recovered from the RE-cmeABC screening. We detected 122 (11.2%) RE-cmeABC-positive isolates of chicken origin, including 111 (70.7%) C. jejuni and 11 (1.2%) C. coli. This multidrug efflux pump is more prevalent among C. jejuni than C. coli. The level of resistance was significantly different in 111 RE-cmeABC-positive C. jejuni versus 46 RE-cmeABC-negative C. jejuni for florfenicol, clindamycin, and erythromycin (P 0.05), but not for ciprofloxacin (CIP), TET, and gentamicin (GEN). However, the isolates harboring RE-cmeABC could shift the minimum inhibitory concentration distribution to the higher range for CIP and TET. Pulsed-field gel electrophoresis (PFGE) analysis suggested that horizontal transmission might be involved in the dissemination of RE-cmeABC in Shanghai and Guangdong, while clonal expansion was predominant in Shandong. Three isolates shared the indiscriminate PFGE types of RE-cmeABC-positive C. jejuni isolates in Shanghai and Guangdong, and four isolates in Shanghai and Shandong. Our study suggests the possibility of a wide dissemination of RE-cmeABC in Campylobacter of food-animal origin, which would pose a significant threat to public health.