Down-regulation of tyrosinase,TRP-1,TRP-2 and MITF expressions by citrus press-cakes in murine B16 F10 melanoma
Down-regulation of tyrosinase,TRP-1,TRP-2 and MITF expressions by citrus press-cakes in murine B16 F10 melanoma作者机构：Cosmetic Science CenterDepartment of ChemistryJeju National UniversityJeju 690-756Korea Citrus Research StationNational Institute of Horticultural & Herbal ScienceRDAJeju 699-946Korea Jeju TechnoparkAra-1-dongJeju-siJeju 690-121Korea Ohyun High School1 Hwabook-dongJeju-siJeju 690-061Korea LINC AgencyJeju National UniversityAra-1-dongJeju 690-756Korea
出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)
年 卷 期：2013年第3卷第8期
页 面：617-622页
学科分类：081702[工学-化学工艺] 08[工学] 0817[工学-化学工程与技术]
基 金：Supported by the Next-Generation BioGreen 21 Program Rural Development Administration Republic of Korea Grant No.PJ009583002013
主 题：Citrus-press cakes Melanin Melanogenesis Tyrosinase MITF TRP-1 TRP-2
摘 要：Objective:To investigate the suitability of citrus-press cakes,by-products of the juice industry as a source for the whitening agents for cosmetic ***:Ethylacetate extracts of citrus-press cakes(CCE)were examined for their anti-melanogenic potentials in terms of the inhibition of melanin production and mechanisim of melanogenesis by using Western Blot analysis with tyrosinese,tyrosinase-related protein-1(TRP-1),TRP2,and microphthalmia-associated transcription factor(MITF)*** apply the topical agents,citrus-press cakes was investigated the safety in human skin cell *** flavonoid analysis of CCE was also determined by HPLC ***:Results indicated that CCE were shown to down-regulate melanin content in a dose-dependent *** CCE inhibited tyrosinase,TRP-2,and MITF expressions in a dose-dependent *** test the applicability of CCE to human skin,we used MTT assay to assess the cytotoxic effects of CCE on human keratinocyte HaCaT *** CCE exhibited low cytotoxicity at 50μg/*** of the citrus-press cakes for flavonoid contents using HPLC showed varied quantity of rutin,narirutin,and ***:Considering the anti-melanogenic activity and human safety,CCE is considered as a potential anti-melanogenic agent and may be effective for topical application for treating hyperpigmentation disorders.
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