SHY2 as a node in the regulation of root meristem development by auxin, brassinosteroids, and cytokinin

作     者：Taotao Li Xinke Kang Wei Lei Xiuhong Yao Lijuan Zou Dawei Zhang Honghui Lin Taotao Li;Xinke Kang;Wei Lei;Xiuhong Yao;Lijuan Zou;Dawei Zhang;Honghui Lin

作者机构：Ministry of Education Key Laboratory for Bio-Resource and Eco-EnvironmentCollege of Life ScienceState Key Laboratory of Hydraulics and Mountain River EngineeringSichuan UniversityChengdu 610064China School of Life Science and EngineeringHenan University of Urban ConstructionPingdingshan 467044China Ecological Security and Protection Key Laboratory of Sichuan ProvinceMianyang Normal UniversityMianyang 621000China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2020年第62卷第10期

页      面：1500-1517页

核心收录：

学科分类：09[农学] 0903[农学-农业资源与环境] 

基　　金：The work was supported by the National Natural ScienceFoundation of China(31670235) the Development Projectof Transgenic Crops of China(2016ZX08009-003-002) the National Natural Science Foundation of China(31970263 and 31972258) Fundamental Research Fundsfor the Central Universities(SCU2019D013) Applied basicproject of Sichuan Science and Technology Department(2018JY0192) 

主　　题：size auxin roots 

摘      要：In multicellular organisms,the balance be-tween cell division and differentiation determines organ size,and represents a central unknown in devel-opmental *** Arabidopsis roots,this balance is mediated between cytokinin and auxin through a reg-ulatory circuit converging on the IAA3/SHORT HYPO-COTYL 2(SHY2)***,we show that crosstalk between brassinosteroids(BRs)and auxin occurs in the vascular transition zone to promote root meristem *** found that BR increases root meristem size by up-regulating expression of the PINFORMED 7(PIN7)gene and down-regulating expression of the SHY2 *** addition,BES1 could directly bind to the pro-moter regions of both PIN7 and SHY2,indicating that PIN7 and SHY2 mediate the BR-induced growth of the root meristem by serving as direct targets of ***,the PIN7 overexpression and loss-of-function SHY2 mutant were sensitive to the effects of BR and could partially suppress the short-root phenotypes associated with deficient BR ***,BRs could inhibit the accumulation of SHY2 protein in response to *** together,these findings suggest that a complex equilibrium model exists in which regulatory interactions among BRs,auxin,and cytokinin regulate optimal root growth.