Fabrication and characterization of epoxylated zwitterionic copolymergrafted silica nanoparticle as a new support for lipase immobilization

作     者：Ning Chen Chunyu Zhang Xiaoyan Dong Yan Sun Ning Chen;Chunyu Zhang;Xiaoyan Dong;Yan Sun

作者机构：Department of Biochemical Engineering and Key Laboratory of Systems Bioengineering(Ministry of Education)School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300354China 

出 版 物：《Chinese Journal of Chemical Engineering》 (中国化学工程学报（英文版）)

年 卷 期：2020年第28卷第4期

页      面：1129-1135页

核心收录：

学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 081705[工学-工业催化] 07[理学] 0817[工学-化学工程与技术] 08[工学] 070205[理学-凝聚态物理] 080501[工学-材料物理与化学] 080502[工学-材料学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 0702[理学-物理学] 

基　　金：funded by the National Natural Science Foundation of China(21621004) the National Key Research and Development Program of China(2018YFA0900702) 

主　　题：Lipase Immobilization Zwitterionic copolymer Interfacial activation Stabilization 

摘      要：Our previous work proved that the thermal stability of Candida rugosa lipase(CRL)immobilized on zwitterionic polymer(poly(carboxybetaine methacrylate))grafted silica nanoparticle(SNP)was much higher than that on poly(glycidyl methecrylate)(pGMA)grafted SNP,while the latter showed significantly increased *** by the research,we have herein proposed to synthesize copolymers of zwitterionic sulfobetaine methacrylate(SBMA)and GMA for CRL *** copolymers were grafted onto SNP surface at three GMA/SBMA(G/S)molar ratios(G100/S0,G50/S50,G10/S90),followed by the covalent coupling of CRL to the surface *** immobilized CRLs on the corresponding supports were denoted as p(G100-S0)-CRL,p(G50-S50)-CRL and p(G10-S90)-*** enzyme loading increased with the increase of GMA content in the copolymer,while the activity varied with the grafted copolymer *** study proved the improvement of enzyme-substrate affinity after *** comparison to p(G100-S0)-CRL,p(G50-S50)-CRL and p(G10-S90)-CRL presented remarkably enhanced thermal stability and pH tolerance,and p(G10-S90)-CRL showed the highest *** results suggest that the copolymer design is promising for development as a versatile platform for enzyme immobilization.