Systematic investigation of bioorthogonal cellular DNA metabolic labeling in a photo-controlled manner

作     者：Shaokang Jia Shixi Yang Huimin Ji Shuang Peng Kun Chen Zhiyong He Xiang Zhou Shaokang Jia;Shixi Yang;Huimin Ji;Shuang Peng;Kun Chen;Zhiyong He;Xiang Zhou

作者机构：College of Chemistry and Molecular SciencesKey Laboratory of Biomedical Polymers of Ministry of EducationWuhan UniversityWuhan 430072China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2020年第31卷第5期

页      面：1104-1108页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 

基　　金：supported by the National Natural Science Foundation of China(Nos.21432008,91753201 and 21721005) the large-scale instrument and equipment sharing foundation of Wuhan University 

主　　题：DNA metabolic labeling Bioorthogonal chemistry Photocage group Modified nucleoside Cell imaging 

摘      要：Bioorthogonal cleavage and ligation reactions together form one more integrated system about the repertoire of bioorthogonal chemistry,capacitating an array of thrilling new biological *** bond-cleavage type and position of biomolecular remain a great challenge,which determines the metabolic pathway of the targets in living *** we designed two linkages of methylene and carbonyl group attached the N-3 position of the 5-ethynyl-2’-deoxyuridine(EdU)base or the oxygen atom at deoxyribose 3’position to a photocaging group,which would be cleaved by irradiation with 365 nm ultraviolet *** derivatives linked by methylene at the N-3 position had better photodecage efficiency and stability in the absence of *** paper provides a strategy for studying the nucleoside metabolic pathways in cells,which can easily and conveniently evaluate the effect of the position and type of the *** developed strategy affords a reference for controlling spatial and temporal metabolism of small-molecule drugs,allowing direct manipulation of intact cells under physiological conditions.