Research of Genetic Diversity in Seven Kobresia by AFLP in Tibetan Plateau

作     者：ZHENG Hong-mei HU Tian-ming WANG Quan-zhen ZHANG Guo-yun SONG Jiang-hu 

作者机构：College of Resources and Environment Northwest A＆F University Yangling 712100 P.R. China College of Animal Science and Technology Northwest A＆F University Yangling 712100 P.R.China Key Laboratory of Agricultural Molecular Biology of Shaanxi Province Yangling 712100 P.R.China 

出 版 物：《Agricultural Sciences in China》 (中国农业科学（英文版）)

年 卷 期：2009年第8卷第8期

页      面：994-999页

核心收录：

学科分类：09[农学] 

基　　金：supported by the Key International Science and Technology Cooperation Program of China(2006DFA33630) the Key Science and Technology Research Program of Tibet,China(2005011) 

主　　题：Kobresia genetic diversity AFLP 

摘      要：This work analyzed the genetic diversity of Kobresia accessions at the molecular level, and further obtained the necessary information for breeding and germplasm evaluation. Genomic DNA of Kobresia was amplified with four E＋3 and M＋3 primer combinations with AFLP （amplified fragment length polymorphism）. AFLP analysis produced 164 scorable bands, of which 154 （93.96%） were polymorphic. The mean Nei＇s gene diversity index （H） was 0.2430, and the Shannon＇s information index （I） was 0.4012, indicating the abundant genetic diversity of Kobresia. The 11 Kobresia accessions from Tibetan Plateau, China, can be classified into five groups after cluster analysis based on the UPGMA （unweigbted pair group method arithmetic average） method. In general, there was abundant genetic diversity among Kobresia accessions resources, and the genetic coefficient was unrelated to their geographic latitude. Natural habitats influenced genetic differentiation of Kobresia.