单纯神经麻风:聚合酶链反应的诊断价值

作     者：Da Cunha F.M.B. Werneck M.C.M. Scola R.H. Werneck L.C. 牛亚利 

作者机构：Servic.o de Doenc.as Neuromuscu-lares/Neurologia Hospital de Clnicas UniversidadeFederal do Paran'a Rua Gal. Carneiro 181 80060-900 Curitiba Brazil Dr. 

出 版 物：《世界核心医学期刊文摘（神经病学分册）》 (Digest of the World Core Medical Journals:Clinical Neurology)

年 卷 期：2006年第2卷第6期

页      面：51-52页

学科分类：0710[理学-生物学] 1002[医学-临床医学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 100206[医学-皮肤病与性病学] 10[医学] 

主　　题：聚合酶链反应(PCR) 结核样型麻风 神经活检 诊断价值 单纯 PCR分析 患者实施 麻风分枝杆菌 抗酸杆菌 

摘      要：Pure neural leprosy (PNL) is often difficult to diagnose when acid-fast bacilli (AFB) cannot be detected. We undertook the present study to evaluate use of the polymerase chain reaction (PCR) in diagnosing PNL. Fifty-eight patients (41 men and 17 women) suspected of pure neural leprosy (PNL) were examined. Patients were classified as borderline tuberculoid (BT, 40 cases) and polar tuberculoid (TT, 18 cases) types. Nerve biopsy was performed and was positive for AFB in 20 patients (all BT patients), i.e., 34.5%of total cases. DNA was extracted from the nerve biopsy samples and amplified using PCR for a specific repeated sequence of DNA from Mycobacterium leprae. PCR analysis was positive in the nerve samples from 29 patients (50%), 27 of the BT type, and 2 of the TT type patients. Further, PCR analysis was positive in 14 of 38 cases that were negative for AFB by nerve biopsy, of which 12 were of the BT type and 2 the TT type. PCR analysis proved to be a useful method to investigate pure neural leprosy, enabling confir mation of the diagnosis in more than a third of the cases that were negative for AFB by nerve biopsy.