polyketide and benzopyran compounds of an endophytic fungus isolated from Cinnamomum mollissimum:biological activity and structure

作     者：Carolina Santiago Lin Sun Murray Herbert Gibson Munro Jacinta Santhanam 

作者机构：Biomedical Science ProgrammeSchool of Diagnostic and Applied Health SciencesFaculty of Health SciencesUniversiti Kebangsaan MalaysiaJalan Raja Muda Abdul Aziz50300 Kuala LumpurMalaysia Department of ChemistryUniversity of CanterburyPrivate Bag 4800Christchurch 8140New Zealand 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2014年第4卷第8期

页      面：627-632页

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supportted by Universiti Kebangsaan Malaysia.Research University Grant UKM-GUP-SK-08-23-300 Science Fund 02-01-02-SF 0517 

主　　题：Endophytic fungi HPLC bioactivity profiling Cinnamomum mollissimum Antimicrobial 

摘      要：Objective:To study bioactivity and compounds produced by an endophytic Phoma *** isolated from the medicinal plant Cinnamomum ***:Compounds produced by the fungus were extracted from fungal broth culture with ethyl *** was followed by hioaclivity profiling of the crude extract fractions obtained via high performance liquid *** fractions were tested for cytotoxicity to P388 murine leukemic cells and antimicrobial activity against bacteria and pathogenic *** purified from active fractions which showed antibacterial,antifungal and cytotoxic activities were identified using capillary nuclear magnetic resonance analysis,mass spectrometry and admission to AntiMarin ***:Three known compounds,namely 4—hydroxymellein,4,8—dihydroxy—6—melhoxy—3—methyl—3,4-dihydro—1H-isochromen-1—one and 1—(2,6-dihydroxyphenyl) ethanone,were isolated from the *** polyketide compound 4—hydroxymellein showed high inhibitory activity against P388 murine leukemic cells(94.6%) and the bacteria Bacillus sublilis(97.3%).Meanwhile.4,8—dihydroxy-6—melhoxy—3—meth) 1—3,4-dihydro—1H—isochromen—1-one,a benzopyran compound,demonstrated moderate inhibitory activity against P388 murine leukemic cells(48.8%)and the fungus Aspergillus niger(56.1%).The second polyketide compound.1(2,6—dihydroxyphenyl)ethanone was inactive against the tested ***:These findings demonstrate the potential of endophytes as producers ol pharmacologically important compounds,including polyketides which are major secondary metabolites in fungi.