A Potential Anti-cancer Compound Separated from the Chloroform Extract of the Chinese Medicine Formula Shenqi San
作者机构：Department of PharmacyMedical CollegeJianghan UniversityWuhan 430056China Hubei Key Laboratory of Resources and Chemistry of Chinese MedicineHubei University of Chinese MedicineWuhan 430065China Department of DermatologyThe Second Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhou 510220China
出 版 物：《Current Medical Science》 (当代医学科学（英文）)
年 卷 期：2020年第40卷第1期
页 面：138-144页
核心收录：
学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学]
基 金：the Natural Science Foundation of Hubei Province of China(No.2018CFB657) the China Postdoctoral Science Foundation(No.2016M592320) the Doctoral Scientific Research Foundation of Jianghan University(No.08250001)
主 题：Shenqi San separation identification A549 apoptosis
摘 要：This study examined anti-cancer compounds present in the chloroform extract of the Chinese medicine formula Shenqi San(CE-SS).Silica gel column chromatography,Sephadex LH-20,octadecylsilyl(ODS)column chromatography,and high performance liquid chromatography(HPLC)were used to separate the compounds from *** structural formulas of the separated compounds were determined using ID1H and 13C experiments as well as high resolution electrospray ionization mass spectroscopy(HRESIMS).The corresponding results were compared with the reported literature data.A total of six compounds were separated and their structures were identified on the basis of corresponding spectroscopic and physico-chemical *** were Saikogenin F(Ⅰ),Prosaikogenin D(Ⅱ),Prosaikogenin F(Ⅲ),p-sitosterol(Ⅳ),3β,16β,23-trihydroxy-13,28-epoxyurs-11-ene-3-O-β-D-glucopyranoside(V),and methyl ursolic acid(VI).The separated compounds were evaluated in vitro for their inhibitory ability against the proliferation of A549 cells via MTT *** was investigated using Annexin V-FITC/propidium iodide(PI)by flow ***-associated proteins were examined by Western *** the compounds were observed to have inhibitory activities against the proliferation of A549 cells to different *** cytometry showed that compound V increased the proportion of apoptotic A549 cells in a dose-dependent *** blotting showed that compound V increased the expression of Bax,cleaved-caspase-3,cleaved-caspase-9 and cleaved-poly ADP-ribose polymerase(PARP),and decreased the expression of *** results indicated that compound V featured a significant inhibitory effect on A549 cells when compared with other compounds,and it may be considered a potential drug against cancers.
维普期刊数据库