Study on linear bio-structure print process based on alginate bio-ink in 3D bio-fabrication

作     者：Youping Gong Zhikai Bi Xiangjuan Bian Anlei Ge Jingyang He Wenxin Li Huifeng Shao Guojin Chen Xiang Zhang Youping Gong;Zhikai Bi;Xiangjuan Bian;Anlei Ge;Jingyang He;Wenxin Li;Huifeng Shao;Guojin Chen;Xiang Zhang

作者机构：School of Mechanical EngineeringHangzhou Dianzi UniversityHangzhou 310018China Faculty of Science and TechnologyZhejiang International Studies UniversityHangzhouChina National Center for International Joint Research of Micro-Nano Molding TechnologyZhengzhou UniversityZhengzhou 450001China 

出 版 物：《Bio-Design and Manufacturing》 (生物设计与制造（英文）)

年 卷 期：2020年第3卷第2期

页      面：109-121页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 0802[工学-机械工程] 0836[工学-生物工程] 080201[工学-机械制造及其自动化] 

基　　金：The work is financially supported by the National Natural Science Foundation of China(51805475 51675148) 

主　　题：Sodium alginate 3D Bio-fabrication Drop-on-demand inject Printing process simulation Linear bio-structure 

摘      要：Ink-jet printing is a non-impact printing technology in which drops jetted from an orifice onto a designated *** technology can digitally transport fluids containing cells precisely onto desired substrates to construct three-dimensional *** order to obtain the stable uniform droplets,a stream is the key point of this ***,there are so many factors that affect the uniform droplet stream construction process:print parameters,material parameters,control method,etc.A good understanding of the various coupled transport processes that occur during bio-ink impact and spreading on bio-structure can improve the success of *** paper aims to obtain a good linear bio-structure with ink-jet printing ***,a typical droplet deposition process model is constructed;including droplet dynamics impact models and droplet diffusion cap ***,a model of successive droplet overlap,to form linear bio-structures,is ***,the finite element method is used to simulate the droplet impact,collision,and fusion ***,the main influencing factors of the continuous injection printing process,namely the time interval between consecutive droplets and the droplet contact angles,are *** alginate is selected as bio-ink to verify the theory,and it is found that a good linear bio-structure could be obtained if the printing parameters are controlled optimally,i.e.,if the initial contact angle is set as 60 degrees and the trigger frequency is set as 150 *** a proper printing speed and gel coating,a good survival rate of printed cells could be obtained.