Transcriptome profiling reveals phase-specific gene expression in the developing barley inflorescence

作     者：Huiran Liu Gang Li Xiujuan Yang Hendrik N.J.Kuijer Wanqi Liang Dabing Zhang Huiran Liu;Gang Li;Xiujuan Yang;Hendrik N.J. Kuijer;Wanqi Liang;Dabing Zhang

作者机构：Joint International Research Laboratory of Metabolic&Developmental SciencesShanghai Jiao Tong University–University of Adelaide Joint Centre for Agriculture and HealthState Key Laboratory of Hybrid RiceSchool of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghai 200240China School of AgricultureFood and WineThe University of AdelaideSouth Australia 5064Australia 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2020年第8卷第1期

页      面：71-86页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：supported by Australian Research Council(DP170103352) an Australia-China Science and Research Fund Joint Research Centre grant ACSRF48187 Start-up funding(Australia,13114779,62117250)for Dabing Zhang from the School of Agriculture,Food and Wine,The University of Adelaide the Innovative Research Team,Ministry of Education of China the 111 Project(B14016) 

主　　题：Inflorescence meristem Transcriptome Gene expression Hormones Barley 

摘      要：The shape of an inflorescence varies among cereals,ranging from a highly branched panicle in rice to a much more compact spike in barley(Hordeum vulgare L.)and wheat(Triticum aestivum L.).However,little is known about the molecular basis of cereal inflorescence *** profiled transcriptomes at three developmental stages of the barley main shoot apex—spikelet initiation,floral organ differentiation,and floral organ growth—and compared them with those from vegetative seedling *** analyses identified 3688 genes differentially transcribed between the three meristem stages,with a further 1394 genes preferentially expressed in reproductive compared with vegetative *** assembly and Gene Ontology analysis classified these 4888 genes into 28 clusters,revealing distinct patterns for genes such as transcription factors,histone modification,and cell-cycle progression specific for each stage of inflorescence *** also compared expression patterns of VRS(SIX-ROWED SPIKE)genes and auxin-,gibberellic acid-and cytokinin-associated genes between two-rowed and six-rowed barley to describe regulators of lateral spikelet *** findings reveal barley inflorescence phase-specific gene expression,identify new candidate genes that regulate barley meristem activities and flower development,and provide a new genetic resource for further dissection of the molecular mechanisms of spike development.